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Merck

T1763

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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About This Item

UNSPSC Code:
12352204
eCl@ss:
42020142
NACRES:
NA.54
EC Number:
3.4.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00082094
Biological source:
bovine pancreas (trypsin)
Concentration:
≥15 units/mL (packed gel)

Key Documents

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biological source

bovine pancreas (trypsin)

form

buffered aqueous suspension

concentration

≥15 units/mL (packed gel)

extent of labeling

≥15 units per mL packed gel

matrix

cross-linked beaded agarose

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Related Categories

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.

Application

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.
Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2

Other Notes

Insolubilized
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T1763-50UN: + T1763-VAR: + T1763-BULK: + T1763-10UN: + T1763-25UN:

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Certificates of Analysis (COA)

Lot/Batch Number
0000498745
0000498745
0000257103
0000267725
0000257103

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Variable activation of immune response by quinoa (Chenopodium quinoa Willd.) prolamins in celiac disease
Zevallos VF, et al.
American Journal of Clinical Nutrition, 96(2), 337-344 (2012)
John P Hobson et al.
The Journal of biological chemistry, 279(45), 46981-46994 (2004-08-26)
We report the identification and functional analysis of a type II transmembrane serine protease encoded by the mouse differentially expressed in squamous cell carcinoma (DESC) 1 gene, and the definition of a cluster of seven homologous DESC1-like genes within a
G Bolte et al.
Clinica chimica acta; international journal of clinical chemistry, 247(1-2), 59-70 (1996-03-29)
For many years, peptic-tryptic digests of gliadin, known as Frazer's fraction III, have been used in investigations of gliadin effects. Potential contamination by the proteases pepsin and trypsin, however, was not considered. To investigate the influence of contaminating proteases on
Amit Tripathi et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(39), 16799-16804 (2009-10-07)
Increased intestinal permeability (IP) has emerged recently as a common underlying mechanism in the pathogenesis of allergic, inflammatory, and autoimmune diseases. The characterization of zonulin, the only physiological mediator known to regulate IP reversibly, has remained elusive. Through proteomic analysis
S C Bracken et al.
Alimentary pharmacology & therapeutics, 23(9), 1307-1314 (2006-04-25)
In coeliac disease, wheat, barley and rye are traditionally excluded in the gluten-free diet. However, few studies have examined the small intestinal immune response to barley and rye. To investigate the immunogenicity of barley and rye prolamins (hordein and secalin
View All Related Papers

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