Skip to Content
Merck

T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
9023-78-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
MFCD00082080
EC Number:
5.3.1.1 (BRENDA, IUBMB)
Specific activity:
~10,000 units/mg protein

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

type

Type I

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Physical form

Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T2507-2.5MG: + T2507-BULK-N: + T2507-5MG: + T2507-VAR-N: + T2507-10MG: + T2507-BULK: + T2507-.5MG: + T2507-VAR: + T2507-25MG:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
036H8025
060H8110

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Xiao Ma et al.
Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)
Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive
Annett Linge et al.
Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)
To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma
Xiaochen He et al.
Journal of cellular physiology, 237(8), 3317-3327 (2022-05-28)
Hypertension is an important risk factor in the pathogenesis of diastolic dysfunction. Growing evidence indicates that glucose metabolism plays an essential role in diastolic dysfunction. TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to regulate glucose metabolism and heart
Rongchang Yang et al.
Experimental parasitology, 137, 46-52 (2013-12-21)
A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning
Richard W Smith et al.
International journal of radiation biology, 89(2), 118-127 (2012-09-26)
To further evaluate the use of microbeam irradiation (MBI) as a potential means of non-invasive brain tumor treatment by investigating the induction of a bystander effect in non-irradiated tissue. Adult rats were irradiated with 35 or 350 Gy at the
View All Related Papers

Articles

硫酸アンモニウム懸濁液として提供される酵素の使用方法

硫酸アンモニウム懸濁液として提供される酵素の使用方法

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Related Content

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service