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Merck

T2663

Trizma® hydrochloride solution

Molecular Biology, 1 M, BioReagent

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:
NA.25
PubChem Substance ID:
24900064
UNSPSC Code:
12161700
MDL number:
MFCD00012590

Key Documents

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InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

SMILES string

Cl.NC(CO)(CO)CO

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

grade

Molecular Biology

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

7.35-7.45

Quality Level

200

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General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T2663-10L: + T2319-100ML-PW: + T2663-100ML: + T2663-100ML-KC: + T2663-1L-KC: + T2663-BULK: + T2663PROC: + T2663-VAR: + T2663-1L: + T2663-PH: + T2663-EW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000251429
0000251429
0000439760
0000439760
0000423330

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Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
El-Refaie Kenawy et al.
Journal of controlled release : official journal of the Controlled Release Society, 81(1-2), 57-64 (2002-05-07)
Electrospun fiber mats are explored as drug delivery vehicles using tetracycline hydrochloride as a model drug. The mats were made either from poly(lactic acid) (PLA), poly(ethylene-co-vinyl acetate) (PEVA), or from a 50:50 blend of the two. The fibers were electrospun
B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
Pengyi Yang et al.
Cell systems, 8(5), 427-445 (2019-05-13)
Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains
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