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Merck

T2694

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

Key Documents

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BioPerformance Certified, Molecular Biology

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

8.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

Quality Level

200

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General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • as a component of extraction buffer for Plasmodiophora brassicae DNA isolation
  • as a component of resuspension buffer for human genomic DNA fragmented sample
  • for adjusting pH of plasma samples and as a component of reconstitution and neutralization buffer

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T2694-VAR: + T2694-1L: + T2694-500ML: + T2694-100ML: + T2694-PH: + T2694PROC: + T2444-100ML-PW: + T2319-1L-PW: + T2694-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000255507
0000259804
0000328985
0000328986
0000255497

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Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing
ElSharawy A, et al.
BMC Genomics, 13(1), 500-500 (2012)
Olivier Harismendy et al.
Genome biology, 12(12), R124-R124 (2011-12-22)
Ultra-deep targeted sequencing (UDT-Seq) can identify subclonal somatic mutations in tumor samples. Early assays' limited breadth and depth restrict their clinical utility. Here, we target 71 kb of mutational hotspots in 42 cancer genes. We present novel methods enhancing both
Anti-peptide monoclonal antibodies generated for immuno-MRM assays have a high probability of supporting Western blot and ELISA
Schoenherr RM, et al.
Molecular and Cellular Proteomics, mcp-O114 (2014)
Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
Mehrabi S, et al.
Bio-protocol (2018)
Markus Frederik Schliffka et al.
eLife, 10 (2021-04-20)
During the first days of mammalian development, the embryo forms the blastocyst, the structure responsible for implanting the mammalian embryo. Consisting of an epithelium enveloping the pluripotent inner cell mass and a fluid-filled lumen, the blastocyst results from a series
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