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Merck

T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

Synonym(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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About This Item

Empirical Formula (Hill Notation):
C16H20N2 · 2HCl
CAS Number:
64285-73-0
Molecular Weight:
313.27
NACRES:
NA.83
PubChem Substance ID:
24900140
UNSPSC Code:
12352204
EC Number:
264-769-6
MDL number:
MFCD00012961

Key Documents

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Product Name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

form

tablet

mp

≥300 °C

storage temp.

2-8°C

Quality Level

200

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Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
  • anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
  • anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
  • in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T3405-5TAB: + T3405-VAR: + T3405-100TAB: + T3405-BULK: + T3405-50TAB:

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Certificates of Analysis (COA)

Lot/Batch Number
0000495630
0000474886
0000474886
0000495630
0000427553

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Sandra Oloketuyi et al.
Biosensors, 11(12) (2021-12-24)
C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher
CD45 isoform expression is associated with different susceptibilities of human naive and effector CD4+ T cells to respond to IL-4
Stutz A, et al.
European Journal of Immunology, 35(2), 575-583 (2005)
Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients
Alamdari DH, et al.
Clinical Biochemistry, 40(3-4), 248-254 (2007)
C Flohr et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 40(1), 131-142 (2009-09-18)
Observational evidence suggests that infection with helminths protects against allergic disease and allergen skin sensitization. It is postulated that such effects are mediated by helminth-induced cytokine responses, in particular IL-10. We tested this hypothesis in a rural area of central
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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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