Skip to Content
Merck

T5525

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, tablet

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
24900293
UNSPSC Code:
12352204
MDL number:
MFCD00007748
Beilstein/REAXYS Number:
2808541

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

form

tablet

mp

168-171 °C (lit.)

storage temp.

2-8°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T5525-100TAB:4548173951911 + T5525-50TAB:4548173951928 + T5525-BULK: + T5525-VAR:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000328835
0000328835
SLCL0439
SLCG6311
SLCB5353

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Lactobacillus casei LcY decreases milk protein immunoreactivity of fermented buttermilk but also contains IgE-reactive proteins
Wroblewska B, et al.
Food Research International, 83, 95-101 (2016)
Effect of acylation and enzymatic modification on pea proteins allergenicity
Szymkiewicz, A and Jedrychowski, L
Polish Journal of Food and Nutrition Sciences, 58(3) (2008)
Immunoreactive properties of peptide fractions of cow whey milk proteins after enzymatic hydrolysis
Wroblewska B, et al.
Polish Journal of Food and Nutrition Sciences, 39(8), 839-850 (2004)
Simon Z He et al.
Leukemia & lymphoma, 56(5), 1406-1415 (2014-09-25)
Acute myeloid leukemia (AML) blasts express high levels of interlekin-3 (IL-3) receptor-α (CD123). CSL360 is a recombinant, chimeric immunoglobulin G1 (IgG1), anti-CD123 monoclonal antibody (MoAb) that neutralizes IL-3 and demonstrates anti-leukemic activity in vitro. This phase 1 study assessed safety
Ronit Mazor et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(23), 8571-8576 (2014-05-07)
Nonhuman proteins have valuable therapeutic properties, but their efficacy is limited by neutralizing antibodies. Recombinant immunotoxins (RITs) are potent anticancer agents that have produced many complete remissions in leukemia, but immunogenicity limits the number of doses that can be given
View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service