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Merck

T7009

Thrombin from human plasma

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

Synonym(s):

Factor IIa

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About This Item

CAS Number:
9002-04-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
MFCD00082072
EC Number:
3.4.21.5 (BRENDA, IUBMB)
Specific activity:
≥1,000 NIH units/mg protein (E1%/280, 18.3)

Key Documents

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form

lyophilized powder

specific activity

≥1,000 NIH units/mg protein (E1%/280, 18.3)

mol wt

37.4 kDa

impurities

HIV and HBsAg, source material tested negative

UniProt accession no.

P00734

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Quality Level

200

Gene Information

human ... F2(2147)

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General description

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.

Application

Thrombin from human plasma has been:
  • dissolved in CaCl2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production
  • to polymerize fibrin on well plates
  • used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Thrombin is an endolytic serine protease which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor. It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer. Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

target_organs

Respiratory system

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T7009-1KU-PW: + T7009-1KU: + T7009-5KU: + T7009-PH: + T7009-250UN-PW: + T7009-100UN: + T7009-250UN: + T7009-BULK: + T7009-5KU-PW: + T7009-VAR: + T7009-100UN-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000531389
0000531389
0000494139
0000494138
0000494138

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Epidemiology and pathophysiology of cancer-associated thrombosis
Noble S and Pasi J
British Journal of Cancer, 102(S1), S2-S2 (2010)
Integrin alphavbetabgr3-RGDS interaction mediates fibrin-induced morphological changes of glomerular endothelial cells
Xu Q, et al.
Kidney International, 56(4), 1413-1422 (1999)
B J Biemond et al.
Circulation, 96(5), 1612-1615 (1997-10-07)
In many case-control as well as epidemiological studies, increased lipoprotein(a) [Lp(a)] levels are considered to constitute an independent risk factor for premature coronary artery and cerebrovascular disease. Lp(a) resembles an LDL particle with an additional linked protein [apolipoprotein(a), apo(a)], whose
Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly (ethylene glycol) hydrogels
Benavides OM, et al.
Tissue Engineering: Part A, 21(7-8), 1185-1194 (2015)
Signal transduction for proteinase-activated receptor-2-triggered prostaglandin E2 formation in human lung epithelial cells
Kawao N, et al.
Journal of Pharmacology and Experimental Therapeutics, 315(2), 576-589 (2005)
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Articles

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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