Protein A Chromatography Resins
Protein A chromatography is a key purification step for monoclonal antibodies (mAbs), multispecific antibodies, Fc-fusion proteins, and antibody-drug conjugates (ADCs). This highly selective purification step provides the foundation for successful downstream purification.
Amsphere® Protein A resins are now part of our portfolio. Amsphere® resins are well-established Protein A resins for efficient purification of antibodies and their derivatives. This expanded resin portfolio offers more options for you to optimize your process, enhance productivity, and improve economics.
Want to learn more about resin performance over time? Download our application note to see how Amsphere® A+ resin delivers high-capacity mAb capture while maintaining reliable performance through repeated CIP cycles.
Key benefits of our Protein A affinity resins
Our Protein A affinity solutions for antibody and recombinant proteins can enhance your purification performance from early development to commercial manufacturing.
Achieve high productivity with resins engineered for high dynamic binding capacity (DBC) and excellent recovery across multiple cycles of sanitization and regeneration.
Accelerate implementation and reduce risk by taking advantage of extensive technical and applications support for your process development and scale-up.
Backed by the Emprove® Program which provides comprehensive technical, regulatory, and supply information to streamline development and facilitate compliance.
Select your Protein A affinity resin
All our Protein A affinity chromatography resins are available as prepacked columns in a range of sizes or as chromatography media to support development from bench scale screening to full-scale manufacturing.
Ampshere® A+ resin
Amsphere® A+ is a next-generation Protein A resin with outstanding dynamic binding capacity (DBC) at short residence times. Amsphere® A+ resin improves capture productivity, compares favorably with market-leading Protein A resins and is ideal for efficient antibody purification in high titer, fed-batch and continuous processes. The exceptional caustic stability and long cycle lifetime ensure reliable performance across extended resin reuse.
- Base bead: Methacrylic copolymer, ~60 µm average particle size
- DBC: ≥60 mg/mL at 4 min residence time (RT) and 10% breakthrough (BT)
- Caustic stability: DBC of 91% after 300 cycles with 0.1 M NaOH (15 min. contact time), 94% DBC after 24 hours in 1M NaOH
Ampshere® A3 resin
Amsphere® A3 resin is an established platform resin with consistent, reproducible Protein A capture performance. The well-balanced productivity and cost-of-goods profile make it a strong fit for platforms that prioritize operational robustness and scalability. Amsphere® A3 resin is a proven choice for dual sourcing strategies for standard mAbs and antibody derivatives to reduce single-supplier risk and strengthen supply chain resilience.
- Base bead: Methacrylic copolymer, ~60 µm average particle size
- DBC: ≥54 mg/mL at 4 min RT and 10% BT
- Caustic stability: DBC of 91% after 300 cycles with 0.1 M NaOH (15 min. contact time), 50% DBC after 24 hours in 1M NaOH
Eshmuno® A resin
Eshmuno® A resin is high-capacity, caustic stable Protein A resin that delivers reliable capture performance even under the most demanding conditions, including high-aggregate feedstocks, multi-specific molecules, and other complex biological formats. The resin’s strong pressure-flow properties enable operations at high linear flow rates, while the selectivity profile supports effective impurity removal.
- Base bead: Rigid hydrophilic polyvinylether, ~50 µm average particle size
- DBC: 40 – 55 mg/mL at 3-6 min RT and 5 % BT
- Caustic stability: 90 % DBC after 200 cycles in 0.1 M NaOH (10% BT), DBC of 88 % after 200 cycles with 0.15 M Phosphoric Acid (6 min. contact time, 5% BT)
Extensive technical and applications expertise
We understand it can be challenging to find the right resin for your process needs. Whether you’re facing a new purification challenge or are looking to second source a resin to maintain manufacturing continuity, we have options.
Process development and manufacturing support
Partner with our process development scientists and MSAT team at the M Lab™ Collaboration Center to optimize and scale your chromatography operation. Either in person or virtual visit, we can help develop, test or troubleshoot without disrupting your manufacturing schedule.
Frequently asked questions
Which affinity resin is best for monoclonal antibody (mAb) capture?
For mAb capture, our Protein A portfolio offers multiple options depending on process goals:
- Amsphere® Protein A resins for standard and intensified mAb capture workflows
- Eshmuno® A is designed for robust and scalable purification of Fc-containing proteins
Selection depends on process requirements including productivity, operating conditions, and process economics.
I’m planning an intensified mAb capture process. Which affinity resin should I consider?
For intensified processing strategies, resin selection should consider dynamic binding capacity, residence time, pressure-flow characteristics, and productivity requirements. Within the Protein A portfolio, different options are designed to support standard through intensified processing approaches.
What is the difference between Amsphere® and Eshmuno® A Protein A resins?
Both support mAb capture applications but address different process needs. Amsphere® resins include options for process optimization and productivity improvements, while Eshmuno® A is positioned as a proven and scalable Protein A solution with broad operating flexibility
Are affinity chromatography resins suitable for intensified processing?
Yes. Affinity chromatography resins can support traditional, intensified, and continuous process strategies depending on resin characteristics and process objectives.
How do affinity resins help reduce downstream bottlenecks?
Affinity purification can combine high selectivity with efficient impurity removal, helping simplify downstream processing and improve productivity. Optimizing the affinity capture step will improve the productivity of subsequent downstream purification steps.
Can I use the same affinity resin from development through commercial manufacturing?
Many affinity chromatography resins are designed to support scale-up from process development through manufacturing. Selection should consider productivity targets, operating window, and lifecycle requirements.
What support is available to help select an affinity chromatography solution?
Our teams provide technical guidance, application expertise, process support, and documentation to help support development, scale-up, and manufacturing decisions.
Are affinity chromatography resins supported by the Emprove® Program?
Selected chromatography solutions are supported through documentation packages designed to facilitate qualification, risk assessment, and regulatory activities. Availability may vary by product.
How can I request support or discuss my purification challenge?
Use our contact form or connect with your account representative to discuss process requirements and identify suitable affinity chromatography options.
Related webinars
Related articles
- How to Optimize Downstream Process Development for Scale Up to a Multi Column Capture Chromatography System
Move towards Biopharma 4.0 and realize the benefits of multi column capture chromatography for intensified downstream process development.
- What is Multi Column Chromatography?
Multi column chromatography has the benefit of using less resin, increasing productivity, and enabling continuous processing. Learn more in this technical article.
- How to select mAb chromatography resins and membranes
Learn how to select chromatography resins for mAb purification based on capacity, selectivity, operating mode, cleaning stability, scalability, and support.
- Protein A affinity chromatography for superior protein purification
Amsphere® A+ Protein A resin shows higher dynamic binding capacity at short residence times and superior impurity removal than other Protein A resins.
- Amsphere® A3: Platform Protein A resin for mAb purification
Amsphere® A3 Protein A chromatography resin offers comparable dynamic binding capacity, recovery and purification performance to platform resins.
- Amsphere® A3 resin: Consistent mAb purification after CIP
Amsphere® A3 Protein A resin offers high binding capacity, robust performance and extended resin lifetime for higher productivity and lower costs.
- Amsphere® A+ affinity chromatography resin - stability and resin life cycle
Amsphere® A+ Protein A resin shows superior caustic stability and resin life cycle compared to modern Protein A resins.
Related documents
- Guide: Biochromatography portfolio
Bulk resin, membranes and prepacked columns.
- Guide: mAb chromatography purification guide
Chromatography product selection guide.
- Data sheet: Amsphere® A+ Resin
Learn more about our next-generation resin, engineered for superior process efficiency.
- Data sheet: Asmphere® A3 Resin
Proven Protein A solution for reliable purification of antibodies and derivatives.
- Data sheet: Eshmuno® A Resin
Eshmuno® A media is a rigid, high-capacity, acid and alkaline resistant Protein A affinity chromatography media for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies.
- Application note: Detection and quantification of leached protein A from Eshmuno® A Resin
Protein A affinity chromatography is the standard capture method for purification of monoclonal antibodies. As some of the protein A ligand co-elutes with the antibody, this impurity must be removed from the product during subsequent purification steps.
- Tech brief: Citric acid or acetic acid? Understanding the impact of elution buffer on mAb purification process
A concise overview of how choosing between citric acid and acetic acid as elution buffers in Protein A chromatography affects mAb purification and downstream processing.
