SCC131 A2EN Human Endocervical Epithelial Cell Line

≥1X10⁶ cells/vial   
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      Key Applications
      Catalogue NumberSCC131
      DescriptionA2EN Human Endocervical Epithelial Cell Line
      OverviewA2EN is an immortalized human epithelial cell line derived from human primary epithelial cells expanded from an explant of human endocervical tissue. The primary endocervical epithelial cells were immortalized with lentiviral vectors carrying the HPV E6/E7 genes. A2EN cells can generate a transepithelial electrical resistance (TEER) when grown on cell culture inserts and generate differential apical and basolateral cytokine profiles upon stimulation with various toll-like receptor (TLR) agonists.

      A2EN human endocervical epithelial cells are adherent and exhibit a keratinocyte appearance when cultured on plastic and a cobblestone appearance with occasional multilayer regions when grown on cell culture inserts.

      1. Herbst-Kralovetz MM, et al. (2008) Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia. Am J Reprod Immunol 59(3): 212-224.

      2. Buckner LR, et al. 2011) Innate immune mediate profiles and their regulation in a novel polarized immortalized epithelial cell model derived from human endocervix. J Reprod Immunol 92(1-2): 8-20.
      Alternate Names
      • A2EN Cell Line
      Background InformationThe endocervical epithelium is a single cell layer lining the endocervical canal that separates the lower female reproductive tract from the uterus and Fallopian tubes. Endocervical epithelial cells secrete mucus and innate immune mediators that facilitate reproduction as well as protect from pathogen invasion. The endocervix is a major site of transmission for multiple sexually transmitted organisms, such as Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and human immunodeficiency virus (HIV).
      Product Information
      Quality LevelMQ100
      ApplicationA2EN endocervical epithelial cell line can be used to study sexually transmitted pathogen infections, pathogenesis and the innate immune response.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for Epstein-Barr virus, HPV-16, HPV-18, Hepatitis A, C, Herpesvirus type 6, 7, 8 and HIV-1 & 2 viruses by PCR
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information