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MABN852-100UG Anti-APP Antibody, clone 2B3

Anti-APP, clone 2B3, Cat. No. MABN852, is a highly specific mouse monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Immunocytochemistry, Inhibiton Assay, and Western Blotting.

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Research Category: Neuroscience Gene Symbol: APP, A4, AD1 UniProt Number: P05067
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MABN852-100UG
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HELISA, ICC, Inhibition, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN852-100UG
      DescriptionAnti-APP Antibody, clone 2B3
      Alternate Names
      • Amyloid-beta A4 protein
      • ABPP
      • APPI
      • Alzheimer disease amyloid protein
      • Amyloid precursor protein
      • Amyloid-beta precursor protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationAmyloid-beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer's disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Applications
      ApplicationAnti-APP, clone 2B3, Cat. No. MABN852, is a highly specific mouse monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Immunocytochemistry, Inhibiton Assay, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Inhibition
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected APP in ELISA applications (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78; Thomas, R.S., et. al. (2013). Neuroreport. 24(18):1058-61).

      Inhibition Analysis: A representative lot inhibited beta amyloid production in MOG-G-UVW culture media (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78).

      Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78).

      Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Thomas, R.S., et. al. (2013). Neuroreport. 24(18):1058-61).
      Biological Information
      ImmunogenA linear peptide corresponding to 15 amino acids from the C-terminal region of human Amyloid-beta A4 protein.
      Epitopeextracellular domain
      Clone2B3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2B3 specifically detects Amyloid-beta A4 protein in human and murine neurons. It targets an epitope within 15 amino acids including the beta-secretase cleavage site.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight86.94 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in mouse cortical neurons.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP in mouse cortical neurons.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABN852-100UG 04054839460197