Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3

Epitope: 285-304 of extracellular domain of rat ASIC3

Synthetic peptide from the extracellular domain of rat ASIC3 (Catalog Number AG366). (C)TASLDPDDFDPEPSDPLGSP

Detect Acid Sensitive Ion Channel 3 using this Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3 validated for use in IC, IH.

Immunohistochemistry: 1:100

Immunocytochemistry: 1:100

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5927

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Neuroinflammation & Pain

ASIC3 (DRASIC)

Affinity purified immunoglobulin. Liquid in PBS, pH 7.4 with 1% BSA and 0.05% sodium azide.

Maintain at -20°C in undiluted for up to 6 months. Avoid repeated freeze/thaw cycles.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.