数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CP01 Sigma-AldrichAnti-Actin (Ab-1) Mouse mAb (JLA20)

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Anti-Actin (Ab-1) Mouse mAb (JLA20): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
カタログ
Data Sheet
Citations

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
A Broad Range Of Species	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
CP01-1EACN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	1 ea	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody. Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1 Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN Entrez Gene Name: actin, alpha 1, skeletal muscle Hu Entrez ID: 58, 71 (Related Antibodies: CP64) Mu Entrez ID: 11459 ;-; 11465 Rat Entrez ID: 29437 ;-; 287876
Catalogue Number	CP01
Brand Family	Calbiochem®

References
References	Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp. Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

Product Information
Form	Liquid
Formulation	100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Positive control	All animal cell lines
Preservative	≤0.1% sodium azide (actin antibody only, CP01-100UL)
Quality Level	MQ100

Applications
Application References	Immunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
Key Applications	Immunoblotting (Western Blotting) Immunofluorescence
Application Notes	Immunoblotting (1:5000-1:20000) Immunofluorescence (1:10000, see application references) Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Application Comments	Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

Biological Information
Immunogen	chicken cytoskeletal proteins
Immunogen	Chicken
Clone	JLA20
Host	Mouse
Isotype	IgM
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot ascites and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
CP01-1EACN	07790788053697

Documentation

Anti-Actin (Ab-1) Mouse mAb (JLA20) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-Actin (Ab-1) Mouse mAb (JLA20) 試験成績書（CoA）

タイトル	ロット番号
CP01	ロット番号を入力

参考資料

参考資料の概要
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp. Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

カタログ

タイトル
Antibody Sourcebookﾙ Sixth Edition

引用

Title

Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	29-October-2008 JSW
Application	Immunoblotting (1:5000-1:20000) Immunofluorescence (1:10000, see application references) Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Description	Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Background	Cytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
Host	Mouse
Immunogen species	Chicken
Immunogen	chicken cytoskeletal proteins
Clone	JLA20
Isotype	IgM
Species	a broad range of species
Positive control	All animal cell lines
Form	Liquid
Formulation	100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Preservative	≤0.1% sodium azide (actin antibody only, CP01-100UL)
Comments	Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot ascites and freeze (-20°C).
Toxicity	Multiple Toxicity Values, refer to MSDS
References	Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp. Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Citation	Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
Application references	Immunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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