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MABS2140-100UG Anti-Active HGF Antibody, clone t8E4

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      Replacement Information
      Catalogue NumberMABS2140-100UG
      DescriptionAnti-Active HGF Antibody, clone t8E4
      Alternate Names
      • Hepatocyte growth factor
      • Hepatopoietin-A
      • Scatter factor
      • SF
      Background InformationHepatocyte growth factor (UniProt: P14210; also known as Hepatopoietin-A, Scatter factor, SF, HGF) is encoded by the HGF (also known as HPTA) gene (Gene ID: 3082) in human. HGF is secreted glycoprotein that is synthesized with a signal peptide (aa 1-31) that is subsequently cleaved off to generate the mature form that contains an - chain (aa 32-494) and a β-chain (495-728) that are linked by a disulfide bridge. Newly synthesized HGF is a single-chain inactive protein (scHGF) that is proteolytically processed by extracellular proteases between arginine 494 and valine 495 into two-chain HGF (tcHGF). Only the tcHGF has biological activity and activates the MET receptor. HGF contains four Kringle domains (aa 128-206, 211-288, 305-383, and 391-465) and one serine protease (SP) domain (495-721). Clone t8E4 is reported to recognize human HGF and does not recognize mouse HGF. It selectively binds only to tcHGF and not to scHGF. It recognizes an epitope present in the K4-SP region, and that this site is formed only after the cleavage between arginine 494 and valine 495. Hence, this clone can be used to distinguish between active tcHGF and inactive scHGF. (Ref.: Umitsu, M., et al. (2016). Sci. Rep. 6; 33149; Jangphattananont, N., et al. (2019). Int. J. Mol. Sci. 20(12); 2955).
      Product Information
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      ApplicationAnti-Active HGF, clone t8E4, Cat. No. MABS2140, is a mouse monoclonal antibody that detects the active form of hepatocyte growth factor and is tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Active HGF in human liver tissue sections.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Active HGF in Immunoprecipitation applications (Umitsu, M., et. al. (2016). Sci Rep. 6:33149).

      ELISA Analysis: A representative lot detected Active HGF in ELISA applications (Umitsu, M., et. al. (2016). Sci Rep. 6:33149).

      Western Blotting Analysis: A representative lot detected Active HGF in Western Blotting applications (Jangphattananont, N., et. al. (2019). Int J Mol Sci. 20(12):2955).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to K2SP portion of human hepatocyte growth factor (HGF).
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      SpecificityClone tc8E4 is a mouse monoclonal antibody that detects the active form of human hepatocyte growth factor (HCF). It targets an epitope within the K4 and SP domains in the C-terminal half.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HGF
      • HPTA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight83.13 kDa calculated.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Active HGF in human kidney tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      カタログ番号 GTIN
      MABS2140-100UG 04065265085885


      Anti-Active HGF Antibody, clone t8E4 (M)SDS



      Anti-Active HGF Antibody, clone t8E4 試験成績書(CoA)

      Anti-Active HGF, clone t8E4 - 4001606 4001606
      Anti-Active HGF, clone t8E4 - 4021518 4021518
      Anti-Active HGF, clone t8E4 - 4048736 4048736
      Anti-Active HGF, clone t8E4 - 4122596 4122596
      Anti-Active HGF, clone t8E4 - Q3539010 Q3539010


      参考資料の概要Pub Med ID
      Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach
      Nawaphat Jangphattananont 1 , Hiroki Sato 2 3 , Ryu Imamura 4 5 , Katsuya Sakai 6 7 , Yumi Terakado 8 , Kazuhiro Murakami 9 , Nick Barker 10 11 , Hiroko Oshima 12 13 , Masanobu Oshima 14 15 , Junichi Takagi 16 , Yukinari Kato 17 , Seiji Yano 18 19 , Kunio Matsumoto
      Int J Mol Sci  20(12)  2955  2019

      31212972 31212972
      Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies
      Masataka Umitsu 1 , Katsuya Sakai 2 , Satoshi Ogasawara 3 , Mika K Kaneko 3 , Ryoko Asaki 1 , Keiko Tamura-Kawakami 1 , Yukinari Kato 3 , Kunio Matsumoto 2 , Junichi Takagi
      Sci Rep  6  33149  2016

      27608665 27608665