Anti-BCL2L12 Antibody, clone 11B5.1

Bcl-2-like protein 12 (UniProt Q9HB09; also known as Bcl-2-related proline-rich protein, Bcl2-L-12) is encoded by the BCL2L12 (also known as BPR) gene (Gene ID 83596) in human. Bcl-2 family proteins paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1 BH4), whereas the BH3-only members contain only the BH3 amphipathic helix that mediates their interaction with the groove of multi-domain BCL-2 family members. Bcl2-L-12 is an anti-apoptotic protein that shares a lower amino acid similarity with the other Bcl2 family members. It contains a BH2 (a.a. 311-322) domain and a BH3-like domain (a.a. 192-220) that mediate its interaction with Bcl2 and Bcl-xL. Exogenous expression of Bcl2-L-12 with Leu213 or Leu217 mutation in U87MG cell line is reported to result in reactivation of PARP cleavage, caspase-3 and cytochrome c release compared to cells expressing wild-type Bcl2-L-12.

~37 kDa observed. 36.82/36.67 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

GST-tagged recombinant human BCL2L12 internal fragment.

Detect Bcl2L12 using this mouse monoclonal Anti-BCL2L12, clone 11B5.1 Antibody, Cat. No. MABC1022, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BCL2L12 in human pancreas, skeletal muscle, and tonsil tissue sections.

Research Category
Apoptosis & Cancer

Clone 11B5.1 targets an internal epitope present in spliced isoforms 1&3, but absent in isoform 2, of human BCL2L12 reported by UniProt (Q9HB09).

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected BCL2L12 in 10 µg of PC3 human prostate cancer cell lysate.

Concentration: Please refer to lot specific datasheet.

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