Anti-Calpain I Antibody, large subunit, clone P9

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

Calpain-I large subunit from human erythrocyte calpain.

Epitope: large subunit

Immunoblot: 1:1,000 Detects an 80kDa large subunit μ-calpain, and will also detect the subunit in the non-activated proform of the zymogen, showing a band of ~110kDa. WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls. Immunocytochemistry: 1:100 on A431 epidermal carcinoma.

Immunoprecipitation

ELISA: 1:6,000 (against the immunogen)

Optimal working dilutions must be determined by the end user.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry

Anti-Calpain I Antibody, large subunit, clone P9 is an antibody against Calpain I for use in ELISA, IP, WB & IC.

Reacts with human calpain-I (μ-Calpain). P9 does not cross with human placental m-calpain (calpain II). This antibody will immunoprecipitate proteolytically active calpains.

Liquid

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Control
WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls.

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