Anti-Caspase 12 Antibody, prodomain, aa 100-116

Three distinct signaling pathways lead to apoptosis. The death receptor and mitochondrion are the two main pathways which involve caspase-8 and caspase-9, respectively. The endoplasmic reticulum stress is the third apoptotic pathway and involves caspase-12. Localized to the ER but not to cytoplasm or mitochondria, caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer′s disease including γ-secretase presenilin and ß-amyloid precursor protein (APP). Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-ß. Caspase-12 is ubiquitously expressed in mouse tissues.

Epitope: prodomain, aa 100-116

Synthetic peptide corresponding to amino acids 100-116 of murine caspase-12.

Detect Caspase 12 using this Anti-Caspase 12 Antibody, prodomain, aa 100-116 validated for use in WB, IH.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

Western blot: 0.5 μg - 1 μg. Detects a band at ~49-53 kDa. in normal tissue extracts. Caspase-12 is a stress induced caspase localized to the ER and activated upon ER stress. Active process forms are difficult to detect in most non-transfected systems. Not all cells produce caspase-12; mouse L929 fibrosacrcoma cells are an excellent positive control line.

Immunohistochemistry: 2 μg /mL in mouse liver tissue

Mouse brain tissue lysate can be used as a positive control. Caspase 12 is associated with ER membranes and not in cytoplasm or mitochondria. Caspase 12 mediates cytotoxixity induced by amyloid beta.

Optimal working dilutions must be determined by end user.

Antibody recognizes caspase-12 pro-protein in mouse extracts of normal and apoptotic cells. Caspase-12 is predicted to have a large, >120 aa, long prodomain region. Thus this antibody recognizes only the proforms of the enzyme and not the active subunits. Uncleaved (proform) caspase-12 is prominently detected in many tissues. Active forms are difficult to detect expect in transfect cell systems. {Kalai, M et al (2003) 162:457-467}.

ImmunoAffinity Purified

Immunoaffinity chromatography purified antibody supplied in PBS containing 0.02% sodium azide.

Maintain at 2°-8°C for up to one year from date of receipt

Control
Positive: mouse brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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