Anti-Cyclin D1/2 Antibody, clone 5D4

34/36 kDa

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. Cyclin D2 is also a G1 cyclin required for G1 phase progression and is a strong candidate for a proto oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.

Recombinant human cyclin D1

Anti-Cyclin D1/2 Antibody, clone 5D4 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin D1/2 & has been validated in ICC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Recognizes p36 cyclin D1 and p34 cyclin D2; no cross-reactivity with cyclin A, B or D3.

Format: Purified

Protein A purified

Protein A purified IgG2a in 50% storage buffer containing PBS, pH 7.2 and 50% glycerol.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysate from human A431 cells

Replaces: 04-1151

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