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MABT913 Anti-Cytokeratin 19 Antibody, clone TROMA-3

Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABT913
100 μg  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABT913
      DescriptionAnti-Cytokeratin 19 Antibody, clone TROMA-3
      Alternate Names
      • Keratin
      • type I cytoskeletal 19
      • CK-19
      • Keratin-19
      • K19
      Background InformationKeratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Electron Microscopy
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.

      Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

      Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

      Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

      Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).

      Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).
      Biological Information
      ImmunogenPurified intermediate filaments from DM1 (Trophoblatoma) cell line.
      CloneTROMA-3
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone TROMA-3 detects cytokeratin 19 in human and murine cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Krt19
      • Krt1-19
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABT913 04054839315985

      Documentation

      Anti-Cytokeratin 19 Antibody, clone TROMA-3 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Cytokeratin 19 Antibody, clone TROMA-3 試験成績書（CoA）

      タイトルロット番号
      Anti-Cytokeratin 19, clone TROMA-3 3072292
      Anti-Cytokeratin 19, clone TROMA-3 - 3261272 3261272
      Anti-Cytokeratin 19, clone TROMA-3 - 3379915 3379915
      Anti-Cytokeratin 19, clone TROMA-3 - 3698072 3698072
      Anti-Cytokeratin 19, clone TROMA-3 - 3931681 3931681
      Anti-Cytokeratin 19, clone TROMA-3 - 4017746 4017746
      Anti-Cytokeratin 19, clone TROMA-3 - 4052390 4052390
      Anti-Cytokeratin 19, clone TROMA-3 - 4092651 4092651
      Anti-Cytokeratin 19, clone TROMA-3 - 4144886 4144886
      Anti-Cytokeratin 19, clone TROMA-3 - 4219968 4219968
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