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MABT1492-100UG Anti-Desmoplakin Antibody, clone 20B6

Anti-Desmoplakin, clone 20B6, Cat. No. MABT1492, is a mouse monoclonal antibody that detects human Desmoplakin and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-Desmoplakin Antibody, clone 20B6: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Cell Structure Gene Symbol: DSP UniProt Number: P15924
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MABT1492-100UG
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1492-100UG
      DescriptionAnti-Desmoplakin Antibody, clone 20B6
      Alternate Names
      • DP
      • 250/210 kDa paraneoplastic pemphigus antigen
      Background InformationDesmoplakin (UniProt: P15924; also known as DP, 250/210 kDa paraneoplastic pemphigus antigen) is encoded by the DSP gene (Gene ID:1832) in human. Desmoplakin is a major high molecular weight homodimeric protein that is involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes, which are prominent adhesive junctions present between many epithelial cells and cardiomyocytes. Desmoplakin is the most abundant of the desmosomal proteins and plays a key role in tissue morphogenesis and maintaining the integrity of tissue. Desmoplakin is composed of an N-terminal plakin domain, a central alpha-helical coiled-coil rod domain, and a C-terminal intermediate filament-binding domain. The N-terminal domain is required for desmosome association and the C-terminal binds to desmin. Three isoforms for Desmoplakin have been reported that are produced by alternative splicing. Desmoplakin 2 and 3 lack most of the central rod domain. Desmoplakin 1 is the dominant isoform in the cardiac tissue. Desmoplakin is phosphorylated by protein kinase A on serine 2849 and this phosphorylation affects its association with epidermal, simple cytokeratins, and VIM intermediate filaments. Mutations in DSP gene have been linked to DCWHK (Cardiomyopathy, dilated, with woolly hair and keratoderma) that is characterized by a generalized striate keratoderma particularly affecting the palmoplantar epidermis, woolly hair, and dilated left ventricular cardiomyopathy. Mice deficient in Desmoplakin perish around the time of implantation (E6.5) and show fewer desmosomes.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Desmoplakin, clone 20B6, Cat. No. MABT1492, is a mouse monoclonal antibody that detects human Desmoplakin and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).

      Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
      Clone20B6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DSP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight331.77 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Desmoplakin in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABT1492-100UG 04054839557934

      Documentation

      Anti-Desmoplakin Antibody, clone 20B6 試験成績書（CoA）

      タイトルロット番号
      Anti-Desmoplakin, clone 20B6 - 3425418 3425418
      Anti-Desmoplakin, clone 20B6 - 3987719 3987719
      Anti-Desmoplakin, clone 20B6 - 4020049 4020049
      Anti-Desmoplakin, clone 20B6 - 4219971 4219971
      Anti-Desmoplakin, clone 20B6 - 4235340 4235340
      Anti-Desmoplakin, clone 20B6 - 4288924 4288924
      Anti-Desmoplakin, clone 20B6 - 4334041 4334041
      Anti-Desmoplakin, clone 20B6 - 4354803 4354803
      Anti-Desmoplakin, clone 20B6 Monoclonal Antibody Q3099051