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MABE160 Anti-E2F4 Antibody, clone LLF4-2

MABE160
100 µg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE160
      DescriptionAnti-E2F4 Antibody, clone LLF4-2
      Alternate Names
      • Transcription factor E2F4
      • E2F-4
      Background InformationE2F4 is the most common E2F family member in a number of cell types. E2F4 deficiency has been shown to cause a decrease in the thickness of the gut epithelium, decrease in maturation of hematopoietic cell types and an increased rate of apoptosis, suggesting that E2F4 plays a role in normal maturation of several cell types. E2F4 has also been shown to be essential for the normal development of airway epithelium and early eye development. Other data has implicated E2F4 during normal heart development, specifically cardiomyocyte proliferation.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-E2F4 Antibody, clone LLF4-2 is a Mouse Monoclonal Antibody for detection of E2F4 also known as Transcription factor E2F4, E2F-4 & has been validated in WB & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kinross, K., et al. (2006). Blood. 108(3):886-895.)
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human E2F4.
      CloneLLF4-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq].
      Gene Symbol
      • E2F4
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.

      SUBUNIT STRUCTURE: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. The E2F4/DP-1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

      DEVELOPMENTAL STAGE: Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.

      PTM: Differentially phosphorylated in vivo.

      POLYMORPHISM: The poly-Ser region of E2F4 is polymorphic and the number of Ser varies in the population (from 8 to 17). The variation might be associated with tumorigenesis.

      SEQUENCE SIMILARITIES: Belongs to the E2F/DP family.
      Molecular WeightBands may be detected at ~45 and ~60 kDA
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F4 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-E2F4 Antibody, clone LLF4-2 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-E2F4 Antibody, clone LLF4-2 試験成績書(CoA)

      タイトルロット番号
      Anti-E2F4, clone LLF4-2 - 24248122424812
      Anti-E2F4, clone LLF4-2 - 32160023216002
      Anti-E2F4, clone LLF4-2 - 33131403313140
      Anti-E2F4, clone LLF4-2 - NRG1897812NRG1897812
      Anti-E2F4, clone LLF4-2 -25671182567118
      Anti-E2F4, clone LLF4-2 -28470082847008
      Anti-E2F4, clone LLF4-2 -28574792857479
      Anti-E2F4, clone LLF4-2 Monoclonal Antibody3129260
      Anti-E2F4, clone LLF4-2 Monoclonal Antibody2896081

      カタログ

      タイトル
      New Products: Volume 3, 2012

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies