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ABT63 Anti-Ena/VASP-like protein Antibody

Detect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.

Anti-Ena/VASP-like protein Antibody: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: EVL, RNB6 UniProt Number: Q9UI08
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ABT63
100 µg  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, ChpWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT63
      DescriptionAnti-Ena/VASP-like protein Antibody
      Alternate Names
      • Ena/VASP-like protein
      • Ena/vasodilator-stimulated phosphoprotein-like
      Background InformationEna (VASP-like protein) is involved in many different cellular functions including cell migration and axon guidance. Studies have shown that Ena expression is significantly increased in breast cancer and that the level of expression is associated with tumor stage. Ena has also been linked to inner ear and hair cell development.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Ramos cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ena/VASP-like protein in NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the EVH2 region of human Ena/VASP-like protein.
      EpitopeEVH2 region
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Ena/VASP-like protein at the EVH2 region.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chimpanzee
      Species Reactivity NoteDemonstrated to react with Human and Mouse.
      Predicted to react with Rat and Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EVL
      • RNB6
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes (By similarity).

      SUBUNIT STRUCTURE: Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of ZYX.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton (By similarity). Cell projection › lamellipodium (By similarity). Note: Targeted to the leading edge of lamellipodia and the dital tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes (By similarity).

      DOMAIN: The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

      PTM: Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the Ena/VASP family.

      Contains 1 WH1 domain.
      Molecular Weight~46 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Ramos cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Ena/VASP-like protein in 10 µg of Ramos cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABT63 04053252423406