Anti-Gβ5 Antibody

The Gβ5 (GNB5 guanine nucleotide binding protein (G protein), beta 5) is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa. It has also been found that Gβ5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in Gβ1-4.

This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa

Epitope: D1

Unique peptide sequence derived from the D1 blade of the Gβ5 protein.

Optimal working dilutions must be determined by end user.

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Gβ5 Antibody is validated for use in WB for the detection of Gβ5.

Recognizes D1 area of Gβ5.

Format: Purified

Purified

Purified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G protein Gbeta5 on 10 μg of L6 lysates.

Control
L6 Cell Lysate, plasma membranes from rat cerebrum.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB4068

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.