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MABF222 Anti-IKKa (p45) Antibody, clone 881H3

MABF222
100 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P), IP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABF222
      DescriptionAnti-IKKa (p45) Antibody, clone 881H3
      Alternate Names
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • I-kappa-B kinase alpha
      • IKK-A
      • IKK-alpha
      • IkBKA
      • IkappaB kinase
      • Conserved helix-loop-helix ubiquitous kinase
      • I-kappa-B kinase 1
      • IKK1
      • Nuclear factor NF-kappa-B inhibitor kinase alpha
      • NFKBIKA
      • Transcription factor 16
      • TCF-16
      Background InformationThe protein named Inhibitor of nuclear factor kappa-B kinase subunit alpha, or alternatively I-kappa-B kinase alpha, IKK-A, IKK-alpha, IKKa (p45), IkBKA, IkappaB kinase, I-kappa-B kinase 1, IKK1, NF-kappa-B inhibitor kinase alpha, NFKBIKA, or transcription factor 16 (TCF-16) and encoded by the human gene CHUK/IKKA/TCF16 is a serine kinase that plays an essential role in the NF-kappa-B signaling pathway. IKKa(p45) acts as part of a complex that phosphorylates inhibitors of the NF-kappa-B. This allows then the polyubiquitination of the inhibitors and their subsequent degradation. Free NF-kappa-B is translocated into the nucleus and performs its gene activation functions governing everything from the immune response to growth and cell survival. IKKa(p45) can be found expressed in most tissues and localizes to both the cytoplasm and nucleus depending upon the microenvironment. Defects in IKKa(p45) are associated with a lethal fetal syndrome called by Cocoon syndrome where the fetus has multiple malformations including limbs bound to the trunk and encased under the skin.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-IKKa (p45) Antibody, clone 881H3 is validated for use in western blotting, IHC (Paraffin) & IP for the detection of IKKa (p45).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IKKa (p45) in human kidney and tonsil tissues.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated IKKa (p45) from HCT116 cell lysate (Margalef, P., et al. (2012). Cell Rep. 2(40):840-854).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected IKKa (p45) in human intestinal mucosa, adenoma, and carcinoma tissues (Margalef, P., et al. (2012). Cell Rep. 2(40):840-854.).

      Western Blotting Analysis: A previous lot of this antibody detected the truncated p45-IKKa form observed at ~45 kDa in WT MEF cell lysate, but less so in the KO MEFs, and the full length non-denatured protein at ~80-85 kDa. A non-specific band at ~75 kDa is detected.

      Immunoprecipitation Analysis: A previous lot of this antibody immunoprecipiated the truncated p45-IKKa form observed at ~45 kDa.
      Biological Information
      ImmunogenConjugated linear peptide corresponding to human IKKa (p45).
      Clone881H3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody preferentially binds both the p45 fragment of IKKa (~45 kDa) as well as the full-length non-denatured form (~80-85 kDa). This antibody shows better preferential binding to the p45 fragment by immunoprecipitation, and detects both forms of IKKa by western blot.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHUK
      • IKKA
      • TCF16
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~80-85 kDa & ~45 kDa observed. The full length non-denatured protein may be observed at ~80-85 kDa, whereas the truncated p45-IKKa fragment may be observed at ~45 kDa in some lysates. A non-specific band at ~75 kDa may also be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected IKKa (p45) in 10 µg of HCT116 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-IKKa (p45) Antibody, clone 881H3 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-IKKa (p45) Antibody, clone 881H3 試験成績書(CoA)

      タイトルロット番号
      Anti-IKKa (p45), clone 881H3 - 32707143270714
      Anti-IKKa (p45), clone 881H3 - 35600223560022
      Anti-IKKa (p45), clone 881H3 - Q2365772Q2365772

      参考資料

      参考資料の概要Pub Med ID
      A truncated form of IKKα is responsible for specific nuclear IKK activity in colorectal cancer.
      Margalef, Pol, et al.
      Cell Rep, 2: 840-54 (2012)  2012

      概要を表示する
      23041317 23041317

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies