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MABE328 Anti-MeCP2 Antibody, clone 4H7

MABE328
100 µL  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Porcine, R WB, IP, ChIP, ICC, IHC R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE328
      DescriptionAnti-MeCP2 Antibody, clone 4H7
      Alternate Names
      • Methyl-CpG-binding protein 2
      • MeCp-2 protein
      • MeCp2
      Background InformationDNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA. It represses transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue nuclear extract
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis MeCP2 antibody is validated for use in WB, IHC, ICC, ChIP & IP for the detection of the MeCP2 protein
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse whole brain cell lysates (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
      Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
      Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
      Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
      Biological Information
      ImmunogenFull length, strep-tagged N-terminus of rat MeCP2.
      EpitopeC-terminus
      Clone4H7
      HostRat
      SpecificityThis antibody recognizes the C-terminus of MeCP2, which has been epitope mapped between amino acids 310-492.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Porcine
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Porcine and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mecp2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~75 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~55 kDa. The calculated molecular weight is 53 kDa, however MeCP2 has been observed between ~75-100 kDa in western blots (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue nuclear extract.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MeCP2 Antibody, clone 4H7 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-MeCP2 Antibody, clone 4H7 試験成績書(CoA)

      タイトルロット番号
      Anti-MeCP2, clone 4H7 - 23600342360034
      Anti-MeCP2, clone 4H7 - 32609163260916
      Anti-MeCP2, clone 4H7 - Q2109416Q2109416
      Anti-MeCP2, clone 4H7 -26211202621120
      Anti-MeCP2, clone 4H7 -27106212710621
      Anti-MeCP2, clone 4H7 -28445792844579
      Anti-MeCP2, clone 4H7 Monoclonal Antibody3102344

      参考資料

      参考資料の概要Pub Med ID
      Generation and characterization of rat and mouse monoclonal antibodies specific for MeCP2 and their use in X-inactivation studies.
      Jost, KL; Rottach, A; Milden, M; Bertulat, B; Becker, A; Wolf, P; Sandoval, J; Petazzi, P; Huertas, D; Esteller, M; Kremmer, E; Leonhardt, H; Cardoso, MC
      PloS one  6  e26499  2011

      概要を表示する 記事全文
      22140431 22140431

      技術情報

      タイトル
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies