Millipore Sigma Vibrant Logo

ABS2087-25UL Anti-Mff

Anti-Mff, Cat. No. ABS2087, is a rabbit polyclonal antibody that detects Mitochondrial fission factor (Mff) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-Mff: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Signaling Gene Symbol: MFF, C2orf33, AD030, AD033, GL004 UniProt Number: Q9GZY8
View This Product on Sigma-Aldrich
ABS2087-25UL
25 µL  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

       

      お問合せください

      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IF, WBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS2087-25UL
      DescriptionAnti-Mff
      Alternate Names
      • Mitochondrial fission factor
      Background InformationMitochondrial fission factor (UniProt: Q9GZY8; also known as Mff) is encoded by the MFF (also known as C2orf33, AD030, AD033, GL004) gene (Gene ID: 56947) in human. Mff is a single-pass type IV membrane protein that is highly expressed in heart, kidney, liver, brain, muscle, and stomach. Mff is a homodimeric protein, but may also interact with other proteins. It plays a role in mitochondrial and peroxisomal fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. It is also suggested to be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. Mff has a cytoplasmic domain (aa 1-322), a helical domain (aa 323-340), and a mitochondrial intermembrane domain (aa 341-342). Mff is shown to be an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells and its down-regulation inhibits mitochondrial recruitment of Drp1 and induces mitochondria elongation. Also, exogenous expression of Mff is shown to induce extensive mitochondrial fission. Mutations in MFF gene can cause a form of encephalopathy that is characterized by delayed psychomotor development, severe hypotonia with inability to walk, and microcephaly. (Ref.: Otera, H., et al. (2010). J. Cell Biol. 191 (6); 1141 1158; Gandre-Babbe, S., and van der Bliek, AM. (2008). Mol. Biol. Cell. 19(6): 2402-2412).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-Mff, Cat. No. ABS2087, is a rabbit polyclonal antibody that detects Mitochondrial fission factor (Mff) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Mff in Western Blotting applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44).

      Immunofluorescence Analysis: A representative lot detected Mff in Immunofluorescence applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44).
      Biological Information
      ImmunogenHis-taged full length human recombinant mitochondrial fission factor (Mff).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects mitochondrial fission factor (Mff) in human cells.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • MFF
      • C2orf33
      • AD030
      • AD033
      • GL004
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight38.47 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells with Mitotracker (TM).

      Immunocytochemistry Analysis: A 1:2,000 dilution of this antibody detected Mff in HeLa cells with Mitotracker (TM).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABS2087-25UL 04054839543562