MABS1688
Anti-NCoA-2 Antibody, clone 1C6.3
clone 1C6.3, from mouse
別名:
Nuclear receptor coactivator 2, bHLHe75, Class E basic helix-loop-helix protein 75, hTIF2, NCoA-2, Transcriptional intermediary factor 2
ログインで組織・契約価格をご覧ください。
サイズを選択してください
表示を変更する
この商品について
eCl@ss:
32160702
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
1C6.3, monoclonal
Application:
IHC, WB
Citations:
-
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1C6.3, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NCOA2(10499)
General description
Nuclear receptor coactivator 2 (UniProt Q15596; also known as bHLHe75, Class E basic helix-loop-helix protein 75, hTIF2, NCoA-2, Transcriptional intermediary factor 2) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) is a 160 kDa transcriptional coactivator for steroid receptors and nuclear receptors that acts as a coactivator of the steroid binding domain (AF-2), but not the modulating N-terminal domain (AF-1). It possesses a single NR interaction domain (NID) and two autonomous activation domains, AD1 and AD2. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery. Along with NCOA1 it controls energy balance between white and brown adipose tissues and also acts as a critical regulator of glucose metabolism. It is also involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. NCoA-2 is reported to positively regulate the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias.
Ref.: Voegel, JJ et al. (1998). EMBO J. 17, 507 519.
Ref.: Voegel, JJ et al. (1998). EMBO J. 17, 507 519.
~160 kDa observed. 159.0 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST-tagged recombinant human NCoA-2 C-terminal region fragment..
Application
Detect NCOA2 using this mouse monoclonal Anti-NCoA-2 Antibody, clone 1C6.3, Cat. No. MABS1688, validated for use in Immunohistochemistry (Paraffin) and Western blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear NCoA-2 immunoreactivity in human lymphoma, ovarian cancer, and small intestine tissue sections.
Biochem/physiol Actions
Clone 1C6.3 targeted epitope lies within the C-terminal one fourth region of the full-length human NCoA-2.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in human osteosarcoma cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NCoA-2 in 10 µg of U2OS human osteosarcoma cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NCoA-2 in 10 µg of U2OS human osteosarcoma cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Still not finding the right product?
製品選択ツール}で選択肢を絞り込んでください。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MABS1688:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。