Anti-NRF-1 Antibody

Nuclear respiratory factor (UniProt Q9WU00; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the Nrf1 gene (also known as C87038, D6Ertd415e) in murine species (Gene ID 18181). NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS). This results in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

~54/58 kDa observed

KLH-conjugated linear peptide corresponding to mouse NRF-1.

Immunocytochemistry Analysis: A representative lot detected NRF-1 in neonatal rat cardiomyoblasts (H9c2) (Data courtesy of Hagir B. Suliman and Claude A. Piantadosi , Duke University Medical Center).
Western Blotting Analysis: A representative lot detected NRF-1 in rat hepatoma cell line H4IIE treated with t-BH (Suliman, H.B., et al. (2003). J. Biol. Chem. 278(42):41510-41518).
Western Blotting Analysis: A representative lot detected NRF-1 in mouse hepatic nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Western Blotting Analysis: A representative lot detected NRF-1 in mouse liver after S. aureus inoculation (MacGarvey, N.C., et al. (2012). Am J Respir Crit Care Med. 185(8):851-861).
Western Blotting Analysis: A representative lot detected NRF-1 in rat hepatocytes and rat hepatoma cell line H4IIE cells (Carraway, M.S., et al. (2010). Circ Res. 106(11):1722-1730).
Chromatin Immunoprecipitation Analysis: A representative lot detected enhanced NRF-1 occupancy at the Sod2 promoter region in liver nuclear extracts from mice during S. aureus sepsis (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Immunocytochemistry Analysis: A representative lot detected NRF-1 in Staphylococcus aureus peptidoglycan
(PGN) or dichloromethane (DCM)/carbon monoxide (CO) treated HepG2 cells (MacGarvey, N.C., et al. (2012). Am J Respir Crit Care Med. 185(8):851-861).
Immunocytochemistry Analysis: A representative lot detected NRF-1 in H9C2 rat cardiomyocytes (Carraway, M.S., et al. (2010). Circ Res. 106(11):1722-1730).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-NRF-1 Antibody is validated for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP) for the detection of NRF-1 .

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing PBS and 0.01% Sodium Azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected NRF-1 in 10 µg of human liver tissue lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.