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MABS1819-25UG Anti-OTUD3 Antibody, clone 12A6.1

Anti-OTUD3, clone 12A6.1, Cat. No. MABS1819, is a mouse monoclonal antibody that detects OTUD3 in human cells and has been tested for use in Western Blotting.

Anti-OTUD3 Antibody, clone 12A6.1: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Signaling Gene Symbol: OTUD3, KIAA0459 UniProt Number: Q5T2D3
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MABS1819-25UG
25 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1819-25UG
      DescriptionAnti-OTUD3 Antibody, clone 12A6.1
      Alternate Names
      • OTU domain-containing protein 3
      • EC 3.4.19.12
      Background InformationOTU domain-containing protein 3 (UniProt: Q5T2D3; also known as EC: 3.4.19.12, OTUD3) is encoded by the OTUD3 (also known as KIAA0459) gene (Gene ID: 23252) in human. OTUD3 is a cytoplasmic deubiquitinating enzyme that hydrolyzes Lys-6- and Lys-11-linked polyubiquitin and is reported to hydrolyze heterotypic (mixed and branched) and homotypic chains. It has an OUT domain (aa 65-189) and a UBA-like domain (aa 230-270). Its specificity is given by the S1' ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops. The UBA-like domain has no effect on ubiquitin hydrolysis. It displays a thiol-dependent ubiquitin-specific protease activity. It is reported to regulate PTEN stability and suppresses tumorigenesis. OTUD3 transgenic mice exhibit higher levels of the PTEN and are shown to be less prone to tumorigenesis. OTUD3 is believed to regulate PTEN in a manner, which is dependent on its DUB activity. The catalytically inactive mutant C76A does not upregulate PTEN and may even have a downregulating effect. It can remove Lys-6, Lys-11, Lys-27, and Lys-48 types of ubiquitin chain on PTEN. (Ref.: Yuan, L., et al (2015). Nat. Cell Biol. 17(9); 1169-1181).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-OTUD3, clone 12A6.1, Cat. No. MABS1819, is a mouse monoclonal antibody that detects OTUD3 in human cells and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 115 amino acids from the C-terminal half of human OTU domain-containing protein 3.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 12A6.1 detects OTU domain-containing protein 3 in human cells. It iargets an epitope within 115 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • OTUD3
      • KIAA0459
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~48 kDa observed; 45.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human cerebral cortex tissue lysate.
      Western Blotting Analysis: 2 µg/mL of this antibody detected OTUD3 in 10 µg of human cerebral cortex tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABS1819-25UG 04054839543746