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MABE288 Anti-PCNA Antibody, clone PC10

MABE288
200 µg  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE288
      DescriptionAnti-PCNA Antibody, clone PC10
      Alternate Names
      • Proliferating cell nuclear antigen
      • PCNA
      • Cyclin
      Background InformationProliferating cell nuclear antigen (PCNA) is a heterotrimeric nuclear protein that functions as a clamp on replicating DNA. In cases of DNA damage and failure of the DNA repair pathways, PCNA mediates translesion DNA synthesis, a DNA damage tolerance (DDT) pathway, which allows replication to progress in spite of large lesions to DNA which may be caused by replication stress, ionizing radiation, spontaneous DNA damage, among other factors. PCNA primarily recruits low-fidelity DNA polymerase enzymes that are capable of elongating DNA over lesion sites. PCNA is regulated by cycles of ubiquitination, depending on the DDT pathway involved: PCNA is monoubiquitinated by Rad6–Rad18; polyubiquitinated by the Mms2-Ubc13-Rad5; or sumoylated by the Ubc9-Siz1 complex.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody) validated in WB, IHC, IF to detect PCNA also known as Proliferating cell nuclear antigen, PCNA, Cyclin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PCNA in human colon adenocarcinoma tissues and human tonsil tissues.

      Immunofluorescence Analysis: A 1:1,500 dilution from a representative lot detected PCNA in human lymph nodes and in human colon adenocarcinoma cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human PCNA.
      ClonePC10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
      Gene Symbol
      • PCNA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

      SUBUNIT STRUCTURE: Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 AND PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA.

      SUBCELLULAR LOCATION: Nucleus. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

      POST-TRANSLATIONAL MODIFICATION: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.

      MISCELLANEOUS: Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus.

      SEQUENCE SIMILARITIES: Belongs to the PCNA family.
      Molecular Weight~29 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.01 µg/mL of this antibody recognized PCNA in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PCNA Antibody, clone PC10 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-PCNA Antibody, clone PC10 試験成績書(CoA)

      タイトルロット番号
      Anti-PCNA, clone PC10 - 2382198 2382198
      Anti-PCNA, clone PC10 - 2420493 2420493
      Anti-PCNA, clone PC10 - 3168751 3168751
      Anti-PCNA, clone PC10 - 3227952 3227952
      Anti-PCNA, clone PC10 - 3323031 3323031
      Anti-PCNA, clone PC10 - 3490111 3490111
      Anti-PCNA, clone PC10 - 3593754 3593754
      Anti-PCNA, clone PC10 - Q1998580 Q1998580
      Anti-PCNA, clone PC10 -2489997 2489997
      Anti-PCNA, clone PC10 -2717555 2717555

      カタログ

      タイトル
      New Products: Volume 3, 2012

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies