Anti-PL Scramblase 1 Antibody, clone 1A8

Phospholipid scramblase 1 (UniProt O15162; also known as Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1) is encoded by the PLSCR1 (also known as MMTRA1B) gene (Gene ID 5359) in human. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

~35 kDa observed. Uncharacterized band(s) may appear in some lysates.

Recombinant protein corresponding to human PL Scramblase 1.

Anti-PL Scramblase 1 Antibody, clone 1A8 is an antibody against PL Scramblase 1 for use in Western Blotting, Immunohistochemistry, Immunoprecipitation.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PL Scramblase 1 in 10 µg of A549 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PL Scramblase 1 in mouse colon tissue.
Western Blotting Analysis: A representative lot detected PL scramblase 1 in platelets and lung tissue from wild-type and PLSCR3-/-, but not PLSCR1-/-, mice, while little or no PL scramblase 1 was detected in adipocyte or muscle samples from wild-type or the knockout animals (Wiedmer, T., et al. (2004). Proc Natl Acad Sci USA. 101(36):13296-13301).
Western Blotting Analysis: A representative lot detected retroviral-mediated ectopic expression of murine PLSCR1 in SCF-ER-Hoxb8-immortalized myeloid progenitor cells from PLSCR1−/− mice (Chen, C.W., et al. (2011). J Leukoc Biol. 90(2):221-233).
Immunprecipitation Analysis: A representative lot immunoprecipitated PL scramblase 1 from murine bone marrow-derived mast cells (BMMCs) following IgE receptor activation. Tyrosine phosphorylation of PL scramblase 1 was then checked by Western blotting with clone 4G10 (Kassas, A., et al. (2014). PLoS One. 9(10):e109800).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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