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MABE986 Anti-PTB Antibody, clone BB7

MABE986
100 μg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, WB, IP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE986
      DescriptionAnti-PTB Antibody, clone BB7
      Alternate Names
      • Polypyrimidine tract-binding protein 1
      • PTB
      • 57 kDa RNA-binding protein PPTB-1
      • Heterogeneous nuclear ribonucleoprotein I
      • hnRNP I
      • PTB
      Background InformationPolypyrimidine tract-binding protein 1 (UniProt P26599; also known as 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, Heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, PTB, RNA-binding protein) is encoded by the PTBP1 (also known as HNRNP-I, HNRNPI, HNRPI, PTB, PTB2, PTB3, PTB4, PTB-1, PTB-T) gene (Gene ID 5725) in human. The serine–arginine rich (SR) proteins and the heterogeneous nuclear ribonucleoproteins (hnRNPs) are the most typical families of RBPs involved in splicing regulation. SR proteins generally play a positive role in exon recognition by binding exonic and intronic enhancers near the alternative splice sites. On the other hand, hnRNPs often bind to silencer elements and interfere with the recruitment of spliceosome components or positive regulators. PTB, also known as hnRNP I, belongs to an hnRNP family of RNA-binding proteins and is involved in several aspects of cellular mRNA metabolism including splicing, RNA stability, and internal ribosome entry site (IRES)-mediated translation of viral and cellular mRNAs. PTB functions as a splicing repressor by preventing recruitment of U2AF65 or correct base pairing of U2 snRNP, or by affecting the interaction of U1 snRNP with other components of the spliceosome.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PTB Antibody, clone BB7 is validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation for the detection of PTB .
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected PTB in 10 µg of HT-29 and HEK293 cell lysate.
      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PTB in HeLa, HUVEC, and NIH/3T3 cells.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated PTB in HeLa and WERI-1 nuclear extracts. (Chou, M.Y., et al. (2000). Mol Cell. 5(6):949-57).
      Western Blotting Analysis: A representative lot detected PTB in HeLa and WERI-1 nuclear extracts (Sharma, S., et al. (2005). Mol Cell. 19(4):485-496).
      Biological Information
      ImmunogenRecombinant protein human PTB.
      CloneBb7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTBP1
      • PTB
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~57 kDa observed. The target band may appear as a doublet, most likely due to the presence of spliced isoforms. Isoform 1 (57.2 kDa calculated), isoform 2/PTB2 (59.0 kDa calculated), isoforms 3 (59.6 kDa calculated).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected PTB in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PTB Antibody, clone BB7 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-PTB Antibody, clone BB7 試験成績書(CoA)

      タイトルロット番号
      Anti-PTB, clone BB7 - 30114803011480
      Anti-PTB, clone BB7 - 32569943256994
      Anti-PTB, clone BB7 -Q2578161Q2578161
      Anti-PTB, clone BB7 Monoclonal Antibody2909934
      Anti-PTB, clone BB7 Monoclonal Antibody3150351
      Anti-PTB, clone BB7_28403872840387

      参考資料

      参考資料の概要Pub Med ID
      The splicing regulator Rbfox1 (A2BP1) controls neuronal excitation in the mammalian brain.
      Gehman, LT; Stoilov, P; Maguire, J; Damianov, A; Lin, CH; Shiue, L; Ares, M; Mody, I; Black, DL
      Nature genetics  43  706-11  2011

      概要を表示する
      21623373 21623373
      Developmental control of CaV1.2 L-type calcium channel splicing by Fox proteins.
      Tang, ZZ; Zheng, S; Nikolic, J; Black, DL
      Molecular and cellular biology  29  4757-65  2009

      概要を表示する
      19564422 19564422
      An inducible change in Fox-1/A2BP1 splicing modulates the alternative splicing of downstream neuronal target exons.
      Lee, JA; Tang, ZZ; Black, DL
      Genes & development  23  2284-93  2009

      概要を表示する
      19762510 19762510
      Polypyrimidine tract binding protein blocks the 5' splice site-dependent assembly of U2AF and the prespliceosomal E complex.
      Sharma, S; Falick, AM; Black, DL
      Molecular cell  19  485-96  2005

      概要を表示する
      16109373 16109373
      Multisite RNA binding and release of polypyrimidine tract binding protein during the regulation of c-src neural-specific splicing.
      Chou, MY; Underwood, JG; Nikolic, J; Luu, MH; Black, DL
      Molecular cell  5  949-57  2000

      概要を表示する
      10911989 10911989

      技術情報

      タイトル
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      White Paper: Further considerations of antibody validation and usage.