Anti-Podoplanin Antibody, clone NZ1.2

Human podoplanin (T1alpha-2, aggrus and gp36) is a 38 kDa type-1 transmembrane glycoprotein that consists of 162 amino acids, nine of which form the intracellular domain. The highly O-glycosylated extracellular domain contains a sialic acid that is linked to an alpha-2,3 galactose, which forms the main part of the protein’s carbohydrate moieties. It is expressed in a select variety of human tissues and organs, and is also found in lymphatic, but not blood vessel endothelium. While its mode of action remains unclear, podoplanin is suggested to play a critical role in several physiological processes and conditions such as cell migration, cytoskeleton organization, tissue development, and tissue repair, as well as in carcinogenesis and tumor cell invasion.

~25 kDa observed.
4 isoforms are produced by alternative splicing, at molecular weights of ~17 kDa (isoform 1), ~18 kDa (isoform 2), ~25 kDa (isoform 3), and ~25 kDa (isoform 4).

Epitope: PLAG Domain

Protein sequence corresponding to the PLAG domain of human Podoplanin.

Anti-Podoplanin Antibody, clone NZ1.2 is an antibody against Podoplanin for use in WB, IH.

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Podoplanin in human lung adenocarcinoma and colorectal tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Cytoskeleton

This antibody detects Podoplanin at the PLAG domain.

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human lung tissue lysate.

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Podoplanin in 10 µg of human lung tissue lysate.

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