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ABS1484-25UL Anti-RASSF1

Anti-RASSF1, Cat. No. ABS1484, is a rabbit polyclonal antibody tht detects Ras association domain-containing protein 1 in human and rat tissues and has been tested for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blotting.

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Research Category: Signaling Gene Symbol: RASSF1, RDA32 UniProt Number: Q9NS23
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ABS1484-25UL
25 µL  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HFC, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1484-25UL
      DescriptionAnti-RASSF1
      Alternate Names
      • Ras association domain-containing protein 1
      Background InformationRas association domain-containing protein 1 (UniProt: Q9NS23; RASSF1) is encoded by the RASSF1 (also known as RDA32) gene (Gene ID: 11186) in human. RASSF1 serves as a potential tumor suppressor and is shown to be essential for death receptor-dependent apoptosis. It mediates the activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. In association with MOAP1, it promotes Bax conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Eight isoforms of RASSF1 have been described that are produced by alternative promoter usage and alternative splicing. The immunogen sequence used to generate this antibody is conserved in isoforms A, D, E, F and G. Isoform A is ubiquitously expressed and is shown to localize to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Isoform A is reported to interact with CDC20, an activator of the anaphase-promoting complex (APC), resulting in the inhibition of APC activity and mitotic progression. It Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. It also disrupts interactions among MDM2, DAXX and USP7 and thereby contributes to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RASSF1, Cat. No. ABS1484, is a rabbit polyclonal antibody tht detects Ras association domain-containing protein 1 in human and rat tissues and has been tested for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected RASSF1 in Jurkat cells.

      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RASSF1 in human ovarian cancer, human placenta, and human pancreas tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Ras association domain-containing protein 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Ras association domain-containing protein 1 in human and rat tissues. It targets an epitope within 14 amino acids from the N-terminal region.
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • RASSF1
      • RDA32
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~39 kDa observed; 39.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat liver tissue lysate.
      Western Blotting Analysis: A 1:500 dilution of this antibody detected RASSF1 in 10 µg of r at liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABS1484-25UL 04054839543623