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MABE1048 Anti-SKI2W Antibody, clone 8D10.2

MABE1048
100 µL  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1048
      DescriptionAnti-SKI2W Antibody, clone 8D10.2
      Alternate Names
      • Helicase SKI2W
      • Helicase-like protein
      • HLP
      • Ski2
      Background InformationHelicase SKI2W (UniProt Q15477; also known as Helicase-like protein, HLP, Ski2) is encoded by the SKIV2L (also known as DDX13, SKI2W, SKIV2, THES2, W) gene (Gene ID 6499) in human. Ski2w is a mammalian homologue of yeast ski2 protein proposed to be involved in RNA processing. The presence of a DEVH-box motif (a.a. 423-426) and its in vitro ATPase/GTPase activity both point to Ski2w being an RNA helicase, although such biological activity has not been demonstrated in vivo. In addition, human Ski2w contains two consecutive leucine zipper motifs commonly involved in protein-protein interaction, as well as an RGD motif (a.a. 238-240) that can potentially interact with cell adhesion molecules. Ski2w is ~140 kDa in size and present in the nucleolus and in the cytoplasm. Ski2w is reported to be associated with ribosomes in an mRNA-independent manner, suggesting a role in protein synthesis regulation. SKIV2L gene mutations have been identified in patients with Tricohepatoenteric syndrome (THES) characterized by intrauterine growth retardation, severe diarrhea in infancy, facial dysmorphism, immunodeficiency, and hair abnormalities, mostly trichorrhexis nodosa.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SKI2W, clone 8D10.2 Antibody, Cat. No. MABE1048, is a highly specific mouse monoclonal antibody that targets SKI2W and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SKI2W in human prostate cancer, pancreas, and skeletal muscle tissue sections.

      Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SKI2W in 10 µg of HepG2 cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant fragment from the C-terminal half of human SKI2W.
      Clone8D10.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8D10.2 targets an internal region C-terminal to the helicase domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SKIV2L
      • DDX13
      • SKI2W
      • SKIV2
      • THES2
      • W
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~140 kDa observed. 137.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected SKI2W in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications