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MABF945 Anti-TAP2 Antibody, clone 429.4

MABF945
100 μg  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABF945
      DescriptionAnti-TAP2 Antibody, clone 429.4
      Alternate Names
      • Antigen peptide transporter 2
      • APT2
      • ATP-binding cassette sub-family B member 3
      • Peptide supply factor 2
      • Peptide transporter involved in antigen processing 2
      • Peptide transporter PSF2
      • Peptide transporter TAP2
      • PSF-2
      • Really interesting new gene 11 protein
      Background InformationAntigen peptide transporter 2 (UniProt Q03519; also known as APT2, ATP-binding cassette sub-family B member 3, Peptide supply factor 2, Peptide transporter involved in antigen processing 2, Peptide transporter PSF2, Peptide transporter TAP2, PSF-2, Really interesting new gene 11 protein) is encoded by the TAP2 (also known as ABCB3, PSF2, RING11, Y1) gene (Gene ID 6891) in human. TAP2 (ABCB3) belongs to the subfamily B of ATP-binding cassette (ABC) transporters, members of which also include TAP1 (ABCB2) and multidrug resistance protein 1 (MDR1, ABCB1, P-gp). TAP is a heterodimeric peptide transporter composed of TAP1 and TAP2 that dimerize via their nucleotide-binding domain (NBD; a.a. 503-510) in a head-to-tail fashion. TAP preferentially mediates the transport of 8- to 16-a.a. peptides into the ER in a process powered by ATP-binding and hydrolysis. TAP1 and TAP2 harbor a specialized N-terminal transmembrane domain (TMD0) that is followed by the TAP half-transporter core module composed of one TMD and one NBD. TMD0 is composed of a 4-TM bundle that serves as the interaction platform for the assembly of the peptide-loading complex (PLC).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-TAP2 Antibody, clone 429.4, Cat. No. MABF945 is validated for use in Immunocytochemistry and Western Blotting for the detection of TAP2.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TAP2 in U2OS human osteosarcoma cells.

      Immunocytochemistry Analysis: A 1:400 dilution from a representative lot detected TAP2 in HeLa, but not in the ST-EMO B-lymphoblastoid cell line derived from a patient with homozygous TAP2 deletion (TAP2-/-) (Courtesy of Peter van Endert, Ph.D., Institut Necker Enfants Malades (INEM), Centre De Medecine Moleculaire, Paris, France).
      Biological Information
      ImmunogenBaculovirus-expressed recombinant human TAP2 C-terminal fragment (van Endert, P.M., et al. (1994). Immunity. 1(6):491-500).
      Clone429.4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 429.4 was subcloned from the original 429.3 hybridoma reported by van Endert, P.M., et al. (1994). Immunity. 1(6):491-500. The epitope lies within TAP2 ABC transporter domain. Clone 429 detected TAP2 in HeLa, but not in the ST-EMO B-lymphoblastoid cell line derived from a patient with homozygous TAP2 deletion (TAP2-/-) (Courtesy of Peter van Endert, Ph.D., Institut Necker Enfants Malades (INEM), Centre De Medecine Moleculaire, Paris, France).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TAP2
      • ABCB3
      • PSF2
      • RING11
      • Y1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~70 kDa observed. 75.66/72.00 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected TAP2 in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications