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MABE1144 Anti-TET1 Antibody, clone 5D6

MABE1144
100 μg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ICC, WB, IP, ELISA R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE1144
      ReplacesABE1034
      DescriptionAnti-TET1 Antibody, clone 5D6
      Alternate Names
      • Methylcytosine dioxygenase TET1
      • CXXC-type zinc finger protein 6
      • Ten-eleven translocation 1 gene protein homolog
      Background InformationMethylcytosine dioxygenase TET1 (EC 1.14.11.n2; UniProt Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also know as Cxxc6, Kiaa1676) gene (Gene ID 52463) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TET1 Antibody, clone 5D6 is an antibody against TET1 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected the endogenous TET1 in murine embryonic stem cell (mESC) lysate, as well as exogenously expressed TET1, but not TET2 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET1 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
      ELISA Anaylsis: Clone 5D6 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET1 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
      Biological Information
      ImmunogenHis-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET1.
      EpitopeRegion between DNA-binding and 2-oxoglutarate-binding domains
      Clone5D6
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 5D6 detected exogenously expressed TET1, but not TET2 or TET3, GST fusion protein (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tet1
      • Cxxc6
      • Kiaa1676
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight219.3 calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in mouse ESCs.

      Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TET1 in mouse embryonic stem cells (ESCs).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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      カタログ番号 説明  
      09-872 Anti-Methylcytosine dioxygenase TET1 Antibody 価格&在庫状況を表示

      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies