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Merck

MAB4360C3

Anti-TRA-1-60 Antibody, clone TRA-1-60, Cy3 conjugate

clone TRA-1-60, from mouse, CY3 conjugate

別名:

TRA160, Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
CY3 conjugate
Clone:
TRA-1-60, monoclonal
Application:
ICC
Citations:
5
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biological source

mouse

Quality Segment

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TRA-1-60, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PODXL(5420)

General description

235/410 kDa calculated
TRA-1-60 is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a neuraminidase sensitive epitope of a high molecular weight glycoprotein that are down-regulated upon differentiation. Recently this antigen has been proposed to be a form of the protein podocalyxin. TRA-1-60, can be detected in the serum of germ cell tumor patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Application

Anti-TRA-1-60 Antibody, clone TRA-1-60, Cy3 conjugate is an antibody against TRA-1-60 for use in ICC.
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-60 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

This antibody recognizes human TRA-1-60.

Physical form

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
H9 human embryonic stem cells
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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保管分類

12 - Non Combustible Liquids

wgk

WGK 2



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