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FCMAB115F Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate

FCMAB115F
100 tests  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB115F
      DescriptionAnti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
      Background InformationHuman embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
      References
      Product Information
      FormatFITC
      Control
      • Pluripotent human embryonic stem (ES) cells
      PresentationPurified mouse monoclonal IgM conjugated to FITC in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Immunocytochemical staining of fixed H9 human ES cells incubated for two hours at 2-8ºC with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
      Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37ºC with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
      Flow Cytometry:
      Antibody dilution for cellular staining:
      • Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
      • Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
      • 5 µL for Guava Flow Cytometer
      • 10 µL for other Flow Cytometry instruments
      Applications
      ApplicationDetect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate validated for use in FC & IC.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Working Solution Cells / test Total Reaction Volume
      5 μl 5 x 105 Cells / 95 μl PBS 100 μl
      10 μl 1 x 106 Cells / 90 μl PBS 100 μl


      Biological Information
      Immunogen - Whole Cells corresponding to the of TRA-1-60.
      CloneTRA-1-60
      HostMouse
      SpecificityThis antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
      No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule
      IsotypeIgM
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on Human. based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TRA-1-60
      Purification MethodAmmonium Sulfate Precipitation
      Molecular Weight~235/410 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size 100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate 試験成績書(CoA)

      タイトルロット番号
      Anti-TRA-1-60, clone TRA-1-60 FITC conjugate - NG1717155 NG1717155
      Anti-TRA-1-60, clone TRA-1-60 FITC conjugate - NG1727931 NG1727931
      Anti-TRA-1-60, clone TRA-1-60 FITC conjugate - NG1741003 NG1741003
      Anti-TRA-1-60, clone TRA-1-60 FITC conjugate - NRG1675590 NRG1675590
      Milli-Mark Anti-TRA-1-60, clone TRA- 1-60 FITC conjugate - 1967270 1967270
      Milli-Mark Anti-TRA-1-60, clone TRA- 1-60 FITC conjugate - 2042803 2042803
      Milli-Mark Anti-TRA-1-60, clone TRA- 1-60 FITC conjugate - 2070904 2070904
      Milli-Mark Anti-TRA-1-60, clone TRA- 1-60 FITC conjugate - 2197267 2197267
      Milli-Mark Anti-TRA-1-60, clone TRA- 1-60 FITC conjugate - 2198086 2198086
      Milli-Mark Anti-TRA-1-60, clone TRA- 1-60 FITC conjugate - NG1786622 NG1786622

      参考資料

      参考資料の概要Pub Med ID
      Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
      Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
      Nature protocols  7  2029-40  2012

      概要を表示する
      23099485 23099485
      Variability in the generation of induced pluripotent stem cells: importance for disease modeling.
      Vitale, AM; Matigian, NA; Ravishankar, S; Bellette, B; Wood, SA; Wolvetang, EJ; Mackay-Sim, A
      Stem cells translational medicine  1  641-50  2012

      概要を表示する
      23197870 23197870