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MAB4381 Anti-TRA-1-81 Antibody, clone TRA-1-81

100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FC, IF, IP, ICCMPurifiedMonoclonal Antibody
      Catalogue NumberMAB4381
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-TRA-1-81 Antibody, clone TRA-1-81
      Background InformationHuman embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.
      Product Information
      • Human embryonic stem cells
      PresentationLiquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
      Quality LevelMQ100
      ApplicationAnti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunofluorescence: A previous lot of this antibody was used in IF.

      Western Blot: A previous lot of this antibody was used in WB.

      Immunoprecipitation: A previous lot of this antibody was used in IP.

      Immunohistochemistry: A previous lot of this antibody was used in IH.

      Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman embryonal carcinoma cell line 2102Ep
      Cloneclone TRA-1-81
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TRA-1-81
      Purification MethodAmmonium Sulfate Precipitation
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight420/250 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-TRA-1-81 Antibody, clone TRA-1-81 (M)SDS



      Anti-TRA-1-81 Antibody, clone TRA-1-81 試験成績書(CoA)

      Anti-TRA-1-81, clone TRA-1-81 2465247
      Anti-TRA-1-81, clone TRA-1-81 - 2375610 2375610
      Anti-TRA-1-81, clone TRA-1-81 - 2430508 2430508
      Anti-TRA-1-81, clone TRA-1-81 - 1991264 1991264
      Anti-TRA-1-81, clone TRA-1-81 - 2019948 2019948
      Anti-TRA-1-81, clone TRA-1-81 - 2074766 2074766
      Anti-TRA-1-81, clone TRA-1-81 - 2191963 2191963
      Anti-TRA-1-81, clone TRA-1-81 - 2282166 2282166
      Anti-TRA-1-81, clone TRA-1-81 - 2625334 2625334
      Anti-TRA-1-81, clone TRA-1-81 - 3218217 3218217


      参考資料の概要アプリケーション動物種Pub Med ID
      Derivation of induced pluripotent stem cells from orangutan skin fibroblasts.
      Ramaswamy, K; Yik, WY; Wang, XM; Oliphant, EN; Lu, W; Shibata, D; Ryder, OA; Hacia, JG
      BMC research notes  8  577  2015

      26475477 26475477
      V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
      Pino-Barrio, MJ; García-García, E; Menéndez, P; Martínez-Serrano, A
      PloS one  10  e0118499  2015

      25764185 25764185
      An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.
      Nishishita, N; Muramatsu, M; Kawamata, S
      American journal of stem cells  4  38-49  2015

      25973330 25973330
      Synaptic dysregulation in a human iPS cell model of mental disorders.
      Wen, Z; Nguyen, HN; Guo, Z; Lalli, MA; Wang, X; Su, Y; Kim, NS; Yoon, KJ; Shin, J; Zhang, C; Makri, G; Nauen, D; Yu, H; Guzman, E; Chiang, CH; Yoritomo, N; Kaibuchi, K; Zou, J; Christian, KM; Cheng, L; Ross, CA; Margolis, RL; Chen, G; Kosik, KS; Song, H; Ming, GL
      Nature  2014

      25132547 25132547
      Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.
      Kishino, Y; Seki, T; Fujita, J; Yuasa, S; Tohyama, S; Kunitomi, A; Tabei, R; Nakajima, K; Okada, M; Hirano, A; Kanazawa, H; Fukuda, K
      PloS one  9  e97397  2014

      24824994 24824994
      Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
      Yamada, Mitsutoshi, et al.
      Nature, (2014)  2014

      24776804 24776804
      Development of a high-yield technique to isolate spermatogonial stem cells from porcine testes.
      Park, MH; Park, JE; Kim, MS; Lee, KY; Park, HJ; Yun, JI; Choi, JH; Lee, Es; Lee, ST
      Journal of assisted reproduction and genetics  31  983-91  2014

      Fluorescence Activated Cell Sorting (FACS), Immunocytochemistry24938360 24938360
      Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
      Tolar, J; McGrath, JA; Xia, L; Riddle, MJ; Lees, CJ; Eide, C; Keene, DR; Liu, L; Osborn, MJ; Lund, TC; Blazar, BR; Wagner, JE
      The Journal of investigative dermatology  134  1246-54  2014

      24317394 24317394
      Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts.
      Whitworth, DJ; Ovchinnikov, DA; Sun, J; Fortuna, PR; Wolvetang, EJ
      Stem cells and development  23  1515-23  2014

      24555755 24555755
      Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
      Bershteyn, M; Hayashi, Y; Desachy, G; Hsiao, EC; Sami, S; Tsang, KM; Weiss, LA; Kriegstein, AR; Yamanaka, S; Wynshaw-Boris, A
      Nature  507  99-103  2014

      Immunofluorescence24413397 24413397


      Human Stem Cell Systems


      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells


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      Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein