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MAB4381C3 Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate

MAB4381C3
100 µL  
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      概要

      Replacement Information

      Special Offers[海外情報]

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMCy3Monoclonal Antibody
      Description
      Catalogue NumberMAB4381C3
      DescriptionAnti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate
      Alternate Names
      • Podocalyxin
      • GCTM-2 antigen
      • Gp200
      • Podocalyxin-like protein 1
      Background InformationTRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin.
      References
      Product Information
      FormatCy3
      Control
      • H9 human embryonic stem cells
      PresentationPurified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81.
      Key Applications
      • Immunocytochemistry
      Application NotesEvaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012).
      Biological Information
      ImmunogenHuman embryonal carcinoma cell line 2102Ep
      Cloneclone TRA-1-81
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes human TRA-1-81.
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PODXL
      • PCLP
      Purification MethodAmmonium sulfate precipitation followed by size exclusion.
      UniProt Number
      Molecular Weight420/250 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in H9 human embryonic stem cells.
      Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate 試験成績書(CoA)

      タイトルロット番号
      Anti-TRA-1-81, clone TRA-1-81, Cy3 -2551800 2551800
      Anti-TRA-1-81, clone TRA-1-81, Cy3 -2594435 2594435
      Anti-TRA-1-81, clone TRA-1-81, Cy3 -2779137 2779137
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - 2448422 2448422
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - Q2038455 Q2038455
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - 2201479 2201479
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - 2208784 2208784
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - 2262702 2262702
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - 2283908 2283908
      Anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate - 3162527 3162527

      参考資料

      参考資料の概要Pub Med ID
      Somatic coding mutations in human induced pluripotent stem cells.
      Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K.
      Nature  471  63-7  2011

      概要を表示する
      21368825 21368825
      A nonviral minicircle vector for deriving human iPS cells.
      Jia, Fangjun, et al.
      Nat. Methods, 7: 197-9 (2010)  2010

      概要を表示する
      20139967 20139967
      Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.
      Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC
      Stem Cells  28  36-44.  2010

      概要を表示する
      19890879 19890879
      The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
      Badcock, G, et al.
      Cancer Res., 59: 4715-9 (1999)  1999

      概要を表示する
      10493530 10493530
      Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
      Andrews, P W, et al.
      Hybridoma, 3: 347-61 (1984)  1984

      概要を表示する
      6396197 6396197

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies