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ABE244-S Anti-monomethyl-Histone H3 (Lys36) Antibody, Trial Size

Anti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications.

Anti-monomethyl-Histone H3 (Lys36) Antibody, Trial Size: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695
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ABE244-S
10 µg  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWB, IP, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE244-S
      DescriptionAnti-monomethyl-Histone H3 (Lys36) Antibody, Trial Size
      Alternate Names
      • H3 histone family, member T
      • Histone 3, H3
      • Histone cluster 3, H3
      • H3K36me1
      Background InformationHistone H3 is one of four histones that form a core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can alter chromatin structure and stability, and influence DNA repair and gene expression. Histone H3 undergoes methylation of its lysine and arginine residues, with varying biological outcomes. The lysine 36 residue undergoes mono-, di-, or trimethylation which may be performed by one of many methyltransferase enzymes (SET2, NSD1, NSD2, NSD3, ASH1, SMYD2, SETMAR, KMT3A, and SETD3). Several studies have indicated that methylated lysine 36 residues promote Rpd3S-mediated deacetylation of other histones resulting in gene activation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract and various recombinant Histone proteins
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Dot Blot
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.

      Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see table) were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 µg/mL).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.
      EpitopeMonomethylated Lys36
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected monomethyl-Histone H3 (Lys36) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABE244-S 04053252907548

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies