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MABE325 Anti-p21WAF1 Antibody, clone EA10

MABE325
100 µg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE325
      DescriptionAnti-p21WAF1 Antibody, clone EA10
      Alternate Names
      • Cyclin-dependent kinase inhibitor 1
      • CDK-interacting protein 1
      • Melanoma differentiation-associated protein 6
      • MDA-6
      • p21
      Background InformationThe tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth.
      References
      Product Information
      FormatPurified
      Control
      • HT-29 cell lysate
      PresentationPurified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-p21WAF1 Antibody, clone EA10 is a Mouse Monoclonal Antibody for detection of p21WAF1 & has been validated in WB, FC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blot Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).

      Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
      Biological Information
      ImmunogenRecombinant protein corresponding to human p21WAF1.
      CloneEA10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDKN1A
      • CAP20
      • CDKN1
      • CIP1
      • MDA6
      • PIC1
      • SDI1
      • WAF1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~21 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HT-29 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-p21WAF1 Antibody, clone EA10 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-p21WAF1 Antibody, clone EA10 試験成績書(CoA)

      タイトルロット番号
      Anti-p21WAF1, clone EA102472963
      Anti-p21WAF1, clone EA10 - 24536882453688
      Anti-p21WAF1, clone EA10 - 25605492560549
      Anti-p21WAF1, clone EA10 - 32182273218227
      Anti-p21WAF1, clone EA10 - 34636293463629
      Anti-p21WAF1, clone EA10 - Q2013509Q2013509
      Anti-p21WAF1, clone EA10 -25563042556304
      Anti-p21WAF1, clone EA10 -26953192695319
      Anti-p21WAF1, clone EA10 -28116352811635
      Anti-p21WAF1, clone EA10 Monoclonal Antibody2900710

      参考資料

      参考資料の概要Pub Med ID
      Detection of p53 dysfunction by flow cytometry in chronic lymphocytic leukaemia.
      Carter, A, et al.
      Br. J. Haematol., 127: 425-8 (2004)  2004

      概要を表示する
      15521919 15521919
      Topological control of p21WAF1/CIP1 expression in normal and neoplastic tissues.
      el-Deiry, W S, et al.
      Cancer Res., 55: 2910-9 (1995)  1995

      概要を表示する
      7796420 7796420

      カタログ

      タイトル
      New Products: Volume 3, 2012

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies