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			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABS1282 Sigma-AldrichAnti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108

Detect GRASP65 using this mouse monoclonal Anti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108, Cat. No. MABS1282. It is used for Immunocytochemistry and Western Blotting.

Anti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）

Research Category: Signaling Gene Symbol: Gorasp1, Grasp65 UniProt Number: O35254

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MABS1282

100 µL

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概要

Replacement Information

主要スペック表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS1282
Description	Anti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108
Alternate Names	Golgi reassembly-stacking protein 1 Golgi peripheral membrane protein p65 Golgi reassembly-stacking protein of 65 kDa GRASP65 pGRASP65
Background Information	Golgi reassembly-stacking protein 1 (UniProt: O35254; also known as Golgi peripheral membrane protein p65, Golgi reassembly-stacking protein of 65 kDa, GRASP65) is encoded by the Gorasp1 (also known as Grasp65) gene in rat. GRASP65 is a Golgi structural protein and mediates cisternae stacking via self-aggregation as well as interactions with other Golgi membrane-docking proteins, such as GM130. Golgi fragmentation results from amyloid beta accumulation, which causes activation of Cdk5 and phosphorylation of GRASP65 resulting in Golgi fragmentation. At least eight GRASP65 phosphorylation sites have been identified. GRASP65 phosphorylation during mitosis and dephosphorylation after mitosis are required for Golgi disassembly and reassembly during the cell cycle. Golgi fragmentation in Alzheimer s disease brains accelerates amyloid precursor protein (APP) trafficking and further enhances amyloid peptide (Ab) production. Expression of non-phosphorylatable mutants of GRASP65 or the inhibition of Cdk5 is shown to rescue the Golgi structure and reduce Ab40 and Ab42 production. GRASP65 mutants are also reported to reduce Ab secretion by increasing alpha cleavage of the APP. (Ref.: Joshi G., et al. (2013). Proc. Natl. Acad. Sci. USA 111(13): E1230-E1239).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect GRASP65 using this mouse monoclonal Anti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108, Cat. No. MABS1282. It is used for Immunocytochemistry and Western Blotting.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blotting Analysis: A representative lot detected phospho-GRASP65 (Thr220/Thr224) in Western Blotting applications (Tang, D., et. al. (2012). Biol Open. 1(12):1204-14; Joshi, G., et. al. (2013). Proc. Natl. Acad. Sci. USA. 111(13):E1230-9). Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-GRASP65 (Thr220/Thr224) in HeLa cells stably expressing rat GRASP65-GFP. Immunocytochemistry Analysis: A representative lot detected phospho-GRASP65 (Thr220/Thr224) in Immunocytochemistry applications (Tang, D., et. al. (2012). Biol Open. 1(12):1204-14). Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-GRASP65 (Thr220/Thr224) in rat liver Golgi and mitotic Golgi fragments.

Biological Information
Immunogen	His-tagged recombinant full-length wild type GRASP65 protein treated with mitotic cytosol.
Clone	LX108
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone LX108 specifically recognizes GRASP65 phosphorylated on Thr220/Thr224.
Isotype	IgG1κ
Species Reactivity	Rat
Species Reactivity Note	Rat. Not Human.
Antibody Type	Monoclonal Antibody
Gene Symbol	Gorasp1 Grasp65
Modifications	Phosphorylation
Purification Method	Protein G purified
UniProt Number	O35254
Molecular Weight	~100 kDa observed. Endogenous band may run at 72 kDa and GFP-tagged will show a band at 100 kDa. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Isotype testing: Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
MABS1282	04054839167492

Documentation

Anti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108 (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-phospho-GRASP65 (Thr220/Thr224) Antibody, clone LX108 試験成績書（CoA）

タイトル	ロット番号
Anti-phospho-GRASP65 (Thr220/Thr224), clone LX108 - 3512483	3512483
Anti-phospho-GRASP65 (Thr220/Thr224), clone LX108 - 3715074	3715074
Anti-phospho-GRASP65 (Thr220/Thr224), clone LX108 - 4047615	4047615
Anti-phospho-GRASP65 -Q2817670	Q2817670

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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