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36-010

Anti-phospho-MKK7/SKK4 (Thr275/Ser277) Antibody

Upstate®, from rabbit

別名:

Dual specificity mitogen-activated protein kinase kinase 7, EC:2.7.12.2, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, MEK 7, Stress-activated protein kinase kinase 4, SKK4, SAPK kinase 4, SAPKK-4, SAPKK4, c-Jun N-terminal kin

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB
Citations:
4
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biological source

rabbit

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), mouse (based on 100% sequence homology)

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_145185.2

UniProt accession no.

O14733

shipped in

dry ice

target post-translational modification

phosphorylation (pThr275/pSer277)

Gene Information

human ... MAP2K7(5609)

General description

Dual specificity mitogen-activated protein kinase kinase 7 (UniProt: O14733; also known as EC:2.7.12.2, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, MEK 7;Stress-activated protein kinase kinase 4, SKK4, SAPK kinase 4, SAPKK-4, SAPKK4, c-Jun N-terminal kinase kinase 2,JNK kinase 2, JNKK 2) is encoded by the MAP2K7 (also known as JNKK2, MEK7, MKK7, RKMK7, SKK4) gene (Gene ID: 5609) in human. MKK4/SKK4 belongs to the STE Ser/Thr protein kinase family and is an essential component of the MAP kinase signal transduction pathway. MKK4/SKK4 is ubiquitously expressed with highest level of expression reported in skeletal muscle. It contains a single protein kinase domain and has a DVD domain (aa 377-400) that contains a conserved docking site and bind to their specific upstream MAP kinase kinase kinases (MAPK3Ks). MKK4/SKK4 also has a D domain in the N-terminal region (aa 37-57), which contains a conserved docking site that is required for the binding of MAPK substrates. It is activated by phosphorylation on Ser271 and Thr275 by MAP kinase kinase kinases (MAP3Ks) and it directly phosphorylates and activates the c-Jun NH2-terminal kinases (JNK), in response to cellular stresses and pro-inflammatory cytokines. Mice lacking the MKK4 gene are shown to have abnormal hepatogenesis and die before embryonic day 14.
~47kDa

Immunogen

peptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4.

Application

Anti-Phospho-MKK7/SKK4 (Thr275/Ser277), Cat. No. 36-010, is a highly specific rabbit polyclonal antibody that detect dual specificity mitogen-activated protein kinase kinase 7 (MKK7) and has been tested for use in Western Blotting.

Biochem/physiol Actions

This rabbit polyclonal antibody detects MKK7/SKK4 phosphorylated on Thr275/Ser277.

Analysis Note

Evaluated by Western Blotting in lysate of A431 cells treates with Calyculin A and okadaic acid.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-MKK7/SKK4 (Thr275/Ser277) in lysate from 10 µg of A431 cells treated with Calyculin A and okadaic acid.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

10 - Combustible liquids

wgk

WGK 1

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36-010:

jan

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