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SCT132 BioTracker 510 Green C2(FM2-10) Synaptic Dye

Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.

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SCT132
5 mg  
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      概要

      Replacement Information
      Description
      Catalogue NumberSCT132
      DescriptionBioTracker 510 Green C2(FM2-10) Synaptic Dye
      OverviewBioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.

      BioTracker 510 Green C2(FM2-10) Synaptic Dye is more water soluble than BioTracker 510 Green C4(FM1-43) Synaptic Dye and thus has a faster de-staining rate.

      Spectral Properties
      Absorbance (MeOH): 510 nm
      Emission (MeOH): 625 nm

      Absorbance (Membranes): 480 nm
      Emission (Membranes): 600 nm
      Alternate Names
      • Live cell imaging probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceSpectral Properties
      Absorbance (MeOH): 510 nm
      Emission (MeOH): 625 nm

      Absorbance (Membranes): 480 nm
      Emission (Membranes): 600 nm
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker 510 Green C2(FM2-10) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.
      Packaging Information
      Material Size5 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      SCT132 04054839566493