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2116 Detergent-OUT™ Med Kit

1 kit  10 columns
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      Key Applications
      Catalogue Number2116
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDetergent-OUT™ Med Kit
      OverviewThe Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. The Med column is suitable for removing detergents from a 1.0 mL solution of 1.5% Triton-X100 detergents (or an equivalent of 15 μL Triton-X100 or 15 mg detergents), provided that both the columns and proteins solutions are prepared in water.
      Materials Required but Not DeliveredCentrifuge

      Centrifuge tubes

      Collection tubes
      Product Information
      • Detergent-OUT Columns-DTG-100X 10 Med
      Key Applications
      • Protein Clean-up
      Application NotesPROTOCOL:

      1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

      2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

      3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

      4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..

      5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
      Packaging Information
      Material Size1 kit
      Material Package10 columns
      Transport Information
      Supplemental Information
      Global Trade Item Number
      カタログ番号 GTIN
      2116 04053252276163


      Detergent-OUT™ Med Kit 試験成績書(CoA)

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      DETERGENT-OUT KIT (MED) - 3321992 3321992
      DETERGENT-OUT KIT (MED) - 3330378 3330378
      DETERGENT-OUT KIT (MED) - 3383279 3383279
      DETERGENT-OUT KIT (MED) - 3429556 3429556
      DETERGENT-OUT KIT (MED) - 3444443 3444443
      DETERGENT-OUT KIT (MED) - 3506772 3506772
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      DETERGENT-OUT KIT (MED) - 3610044 3610044