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イモビロン(Immobilon) トランスファーメンブレンシリーズ

Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.

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Immobilon® Membranes, Sandwiches and Blotting Filter Paper並べ替えとフィルターをクリアする Show Filter
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IPVH07850イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 7 x 8.4 cm シート 7 cm x 8.4 cm 50 価格&在庫状況を表示
IPVH08100イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 8 x 10 cm シート 8 cm x 10 cm 10 価格&在庫状況を表示
IPVH08130イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 8.5 x 13.5 cmシート 8.5 cm x 13.5 cm 10 価格&在庫状況を表示
IPVH09120イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 9 x 12 cm シート 9 cm x 12 cm 10 価格&在庫状況を表示
IPVH10100イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 10 x 10 cm シート 10 cm x 10 cm 10 価格&在庫状況を表示
IPVH15150イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 15 x 15 cm シート 15 cm x 15 cm 10 価格&在庫状況を表示
IPVH20200イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 20 x 20 cm シート 20 cm x 20 cm 10 価格&在庫状況を表示
IPVH304F0イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 26 x 26 cm シート 26 cm x 26 cm 10 価格&在庫状況を表示
IPVH00010イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 26.5 cm x 3.75 m ロール 26.5 cm x 3.75 m 1 価格&在庫状況を表示
ISEQ07850イモビロン(Immobilon)-PSQ メンブレン, PVDF, 0.2 µm, 7 x 8.4 cm シート 7 cm x 8.4 cm 50 価格&在庫状況を表示
ISEQ08100イモビロン(Immobilon)-PSQ メンブレン、 PVDF, 0.2 µm, 8 x 10 cm シート 8 cm x 10 cm 10 価格&在庫状況を表示
ISEQ08130イモビロン(Immobilon)-PSQ メンブレン、 PVDF, 0.2 µm, 8.5 x 13.5 cm シート 8.5 cm x 13.5 cm 10 価格&在庫状況を表示
ISEQ09120イモビロン(Immobilon)-PSQ PVDF 0.2 µm 9 x 12 cm シート 9 cm x 12 cm 10 価格&在庫状況を表示
ISEQ10100イモビロン(Immobilon)-PSQ メンブレン, PVDF, 0.2 µm, 10 x 10 cm シート 10 cm x 10 cm 10 価格&在庫状況を表示
ISEQ15150イモビロン(Immobilon)-PSQ メンブレン, PVDF, 0.2 µm, 15 x 15 cm シート 15 cm x 15 cm 10 価格&在庫状況を表示
ISEQ20200イモビロン(Immobilon)-PSQ メンブレン, PVDF, 0.2 µm, 20 x 20 cm シート 20 cm x 20 cm 10 価格&在庫状況を表示
ISEQ26260イモビロン(Immobilon)-PSQ メンブレン, PVDF, 0.2 µm, 26 x 26 cm シート 26 cm x 26 cm 10 価格&在庫状況を表示
ISEQ00010イモビロン(Immobilon)-PSQ メンブレン, PVDF, 0.2 µm, 26.5 cm x 3.75 m ロール 26.5 cm x 3.75 m 1 価格&在庫状況を表示
IPFL10100イモビロン(Immobilon)-FL PVDF, 0.45 µm, 10 x 10 cm シート 10 cm x 10 cm 10 価格&在庫状況を表示
IPFL20200イモビロン(Immobilon)-FL PVDF, 0.45 µm, 20 x 20 cm シート 20 cm x 20 cm 10 価格&在庫状況を表示
IPFL00010イモビロン(Immobilon)-FL PVDF, 0.45 µm, 26.5 cm x 3.75 m ロール 26.5 cm x 3.75 m 1 価格&在庫状況を表示
IPSN07852ブロッティング濾紙にイモビロン(Immobilon)-P メンブレンを挟みこんだブロッティングサンドイッチ 7 x 8.4 cm 7 cm x 8.4 cm 20 価格&在庫状況を表示
IPSN08132ブロッティング サンドイッチ ブロッティングペーパーではさんだイモビロン(Immobilon)-P メンブレン 8.5 x 13.5 cm 8.5 cm x 13.5 cm 20 価格&在庫状況を表示
IBFP0785Cイモビロン(Immobilon) ブロッティングフィルターペーパー 7 x 8.4 cm sheet 7 cm x 8.4 cm 100 価格&在庫状況を表示
IBFP0813CImmobilon ブロッティングフィルターペーパー、8.5 x 13.5 cm シート 8.5 cm x 13.5 cm 100 価格&在庫状況を表示
IPFL00005Immobilon-FL PVDF Membrane 26.5 cm x 1.875 m 1 価格&在庫状況を表示
IPVH00005Immobilon-P PVDF Membrane 26.5 cm x 1.875 m 1 価格&在庫状況を表示
ISEQ00005Immobilon-PSQ PVDF Membrane 26.5 cm x 1.875 m 1 価格&在庫状況を表示
IEVH00005Immobilon-E PVDF Membrane 26.5 cm x 1.875 m 1 価格&在庫状況を表示
IEVH07804Immobilon-E PVDF Membrane 7 cm x 8.4 cm 4 価格&在庫状況を表示
IEVH07850Immobilon-E PVDF Membrane 7 cm x 8.4 cm 50 価格&在庫状況を表示
IEVH08100Immobilon-E PVDF Membrane 8 cm x 10 cm 10 価格&在庫状況を表示
IEVH09120Immobilon-E PVDF Membrane 9 cm x 12 cm 10 価格&在庫状況を表示
IEVH10100Immobilon-E PVDF Membrane 10 cm x 10 cm 10 価格&在庫状況を表示
IESN07852Immobilon-E Blotting Sandwich 7.0 cm x 8.4 cm 20 価格&在庫状況を表示
IESN08132Immobilon-E Blotting Sandwich 8.5 cm x 13.5 cm 20 価格&在庫状況を表示
IMDISPImmobilon NOW Dispenser 1 価格&在庫状況を表示
IPVH85RImmobilon-P Membrane, PVDF, 0.45 µm, 8.5 cm x 10 m roll 8.5 cm x 10 m 1 価格&在庫状況を表示
ISEQ85RImmobilon-PSQ Membrane, PVDF, 0.2 µm, 8.5 cm x 10 m roll 26.5 cm x 3.75 m 1 価格&在庫状況を表示
IEVH85RImmobilon-E PVDF Membrane 8.5 cm x 10 m 1 価格&在庫状況を表示
IPFL07810Immobilon-FL PVDF Membrane 7 cm x 8.4 cm 10 価格&在庫状況を表示

*Roll

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Documentation

カタログ

タイトル
Western Blotting Tools

技術情報

タイトル
Protein Blotting Handbook: 6th Edition (Merck)
Protein Dot Blotting using Immobilon-P

データシート

タイトル
Immobilon Transfer Membranes: For superior protein and nucleic acid blots

Posters

タイトル
History of Western Blotting
Low Background Membrane for Fluorescent Protein Detection in Western Blotting

参考資料

参考資料の概要アプリケーション
Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities
Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923
LCGC  2010

概要を表示する 記事全文
Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
Luo S., Wehr N.B., Levine R.L.
Analytical Biochemistry:350 (2006):233-238  2006

Immunoblotting (Western)
Pre-B-cell colony-enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium.
Ognjanovic S, Ku TL, Bryant-Greenwood GD.
Am J Obstet Gynecol. 2005 Jul;193(1):273-82  2005

Western Blotting
Role of the Small Heat Shock Proteins in Regulating Vascular Smooth Muscle Tone
McLemore E.C., Tessier D.J., Thresher J., Komalavilas P., Brophy C.M
J. Am. Coll. Surg. 2005, Vol 201 (1):30-36  2005

A high-affinity reversible protein stain for Western blots
Antharavally B.S., Carter, B., Bell, P.A., Mallia K.
Analytical Biochemistry 2004,Vol 329:276-280  2004

Biochemical analysis of GABA receptor subunits alpha 1, alpha 5, beta1 beta2 in the hippocampus of patients with Alzheimer's disease neurophathology
Rissman, R.A., Mishizen-Eberz A.J. N.,Wolfe, C.B.B., DeBlas A.L., Miralles C.P., Ikonomovic M.D., armstrong D.M.
Neuroscience 120 (2003) 295-705  2003

Western Blotting
Proteomics reveals protein profile changes in doxorubicin treated MCF7 human breast cancer cells
Cheng S.T., Pan T, L., Tsai Y.Ch, Huang C. M.
Cancer letters 2002. vol 181:95-107  2002

Towards proteome-wide production of monoclonal antibody by phage display.
Bin Liu, Lan Huang, Carina Sihlbom, Al Burlingame and James D. Marks.
J Mol Biol. 2002 Feb 1;315(5):1063-73  2002

Mass Spectrometry Sample Prep
Amino Acid Analysis: A comparative Study of stains on PVDF membranes
,Journal of Biomolecular Techniques, Vol II, Issue 1, March 2000.
Journal of Biomolecular Techniques, Vol II, Issue 1, March 2000.  2000

Characterization of retinoic acid receptor-deficient keratinocytes.
Goyette Philippe; Chen Chang Feng; Wang Wei; Seguin Francois; Lohnes David(a)
Journal of Biological Chemistry v 275 pg 16497-16505 June 2, 2000  2000

FAQ(よくある質問と回答)

質問回答
Should I prewet MultiScreen Immobilon-P plates before use?Yes. Use 15 ul of 70% ethanol or methanol to prewet the Immobilon P membrane. This membrane is hydrophobic and requires a prewet to allow liquid to pass through the membrane.
What is the thickness of the Immobilon PSQ?Immobilon PSQ is 200 microns thick.
What is the smallest size of a protein or peptide which binds to the Immobilon-PSQ?For the PSQ we do not suggest that they go lower than 2 kDa for the protein size. To help promote the transfer of the smaller proteins the methanol concentration of the transfer buffer can be increased to 20% (w/v) and the SDS lowered to 0.01%. The strength of the electric field can also be reduce by 50% to increase the proteins contact time with the membrane. Standard stains can be used such as Coomasie Blue and Ponceau. Keep in mind that some of the stain such as Coomasie Blue are not reversible.
How many times can I strip and reprobe Immobilon-P?While 2-3 times is probably the limit, it is difficult to give an absolute number in regards to stripping and reprobing. This is because there are many factors to consider when it comes to protein binding to PVDF and primary antibody affinity to these proteins. Different proteins will have varying degrees of affinity to PVDF. This is based on factors discussed in our Protein Blotting Handbook (see TP001; Protein Binding section). One round of stripping may remove one protein and leave another intact. The same could occur when using different primary antibodies. Different antibodies will have varying affinities to different proteins. If carrying out several rounds of stripping and reprobing, one strategy might be to detect the least abundant proteins earlier leaving the higher abundant proteins later for detection.
What is the difference between Immobilon–FL and Immobilon-P?Both membranes are made from PVDF. The difference in background fluorescence is due to proprietary modifications in the membrane manufacturing process.
What fluorescence-based detection methods can be used with Immobilon-FL?Immobilon-FL can be used in Western blotting applications using either fluorescent dye-conjugated antibodies or chemi-fluorescence substrates. Western blots can be imaged in the visible or IR range. Single color detection or multiplexing for co-localization studies can be performed efficiently on Immobilon-FL Fluorescent proteins, e.g. green fluorescent protein, (GFP) or proteins tagged with GFP, blotted onto the membrane can be readily detected as well.
What fluorescent dyes are recommended to use with Immobilon-FL?Immobilon-FL membrane exhibits very low background fluorescence over a broad range of wavelengths and can be used with all visible and infrared fluorescent dyes. Generally, dyes with larger Stokes shift (greater separation between absorption and emission wavelengths) are preferred in western blotting applications using fluorescence-based detection. NOTE: To prevent photo-bleaching, protect the membrane from light during secondary antibody incubations and washes until the membrane is ready to be scanned.
How long does the fluorescent signal stay on the developed blot?The fluorescent signal from fluorescence-tagged antibodies will remain stable on the membrane for several months, or longer, when stored protected from light. The membranes may be stored dry or in PBS at 4ºC. The fluorescent signal from chemi-fluorescent substrates does not remain for long so blots using these substrates need to be scanned immediately.
Do I need to dry Immobilon-FL blots before scanning?Drying the blot may enhance signal strength. NOTE: Do not wrap the blot in plastic/saran wrap while scanning. If you do need to wrap the membrane, Mylar should be used.
Can Immobilon-FL be stripped and re-probed?Yes, Immobilon-FL can be stripped and re-probed like any other PVDF membrane, using standard detergent or acidic methods (refer to Millipore’s Protein Blotting Handbook). Keep the blot wet. If dried, rewet with methanol and then rehydrate before stripping the blot. Once the membrane has dried the stripping is ineffective. Alternatively, the blot can be photo-bleached to remove the fluorescence.

ユーザーガイド

タイトル
Immobilon -P Blotting Sandwiches
Immobilon®-E Transfer Membrane User Guide
Immobilon®-FL Transfer Membrane User Guide
Immobilon®-P Transfer Membrane User Guide
Immobilon®-PSQ Transfer Membrane User Guide
Re-Blot Plus Western Blot Recycling Kit