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17-126 PTP Assay Kit 2

PTP Assay Kit 2 can be used to detect PTP-1B activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.

PTP Assay Kit 2: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Key Applications: Phosphatase Assay
17-126
1 kit  
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      概要

      Replacement Information

      主要スペック表

      Key ApplicationsAnalytes AvailableDetection Methods
      PAPTP Colorimetric
      Description
      Catalogue Number17-126
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPTP Assay Kit 2
      References
      Product Information
      Components
      • Tyrosine Phosphopeptide (TSTEPQpYQPGENL) (Cat.# 12-218)
      • Malachite Green Solution A (Cat.# 20-105)
      • Malachite Green Additive (Cat.# 20-104)
      • Phosphate Standard (Cat.# 20-103)
      • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
      • pNPP Tyr Assay Buffer (Cat.# 20-180)
      • 96-well microtiter plate
      Detection methodColorimetric
      Quality LevelMQ100
      Applications
      ApplicationPTP Assay Kit 2 can be used to detect PTP-1B activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Analytes Available
      • PTP
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • PTPN1
      • PTP-1B
      • PTP1B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
      SIZE: 435 amino acids; 49967 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
      PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
      SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe components in this kit were used to detect PTP-1B (Catalog # 14-109) activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      17-126 04053252407222

      Documentation

      PTP Assay Kit 2 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      PTP Assay Kit 2 試験成績書（CoA）

      タイトルロット番号
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2459578 2459578
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2970636
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2927702
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 3030840
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2896282
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2958930
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2905533
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 3125647
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2197520 2197520
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2279221 2279221
      Show more

      参考資料

      参考資料の概要アプリケーションPub Med ID
      The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes
      Ambach, A., et al
      Eur J Immunol, 30:3422-31 (2000)  2000

      		Phosphatase Assay11093160 11093160
      Phosphorylated synthetic peptides as tools for studying protein phosphatases
      Pinna, L. A. and Donella-Deana, A.
      Biochim Biophys Acta, 1222:415-31 (1994)  1994

      		Phosphatase Assay8038211 8038211
      Inorganic and organic phosphate measurements in the nanomolar range.
      Van Veldhoven, P P and Mannaerts, G P
      Anal. Biochem., 161: 45-8 (1987)  1987

      概要を表示する       		Phosphatase Assay3578786 3578786

      FAQ（よくある質問と回答）

      質問回答
      I still have material left to run more assays, but I need another plate. Where can I find a comparable plate?To get a comparable plate, you can purchase a Costar Brand 96-Well Half Area Plate (for Tissue Culture Section).

      関連製品＆アプリケーション

      Kit Component

      カタログ番号 説明  
      12-218 Tyrosine Phosphopeptide (TSTEPQpYQPGENL) 価格＆在庫状況を表示
      20-103 Phosphate Standard 価格＆在庫状況を表示
      20-104 Malachite Green Additive 価格＆在庫状況を表示
      20-105 Malachite Green Solution A 価格＆在庫状況を表示
      20-106 pNPP (p-Nitrophenyl Phosphate) 価格＆在庫状況を表示
      20-180 pNPP Tyr Assay Buffer 価格＆在庫状況を表示

      カテゴリー

      Life Science Research > Protein Detection and Quantification > Activity Assays > Phosphatase Assays