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71772 ProteoExtract® Transmembrane Protein Extraction Kit

71772
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概要

Replacement Information

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カタログ番号 在庫状況包装 Qty/Pk 価格 数量
71772-3CN
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      20 rxn
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      Description
      OverviewThe ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
      Catalogue Number71772
      Brand Family Novagen®
      References
      ReferencesNakamura, E., et al. 2008. Cancer Sci. 99, 1390.
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 25-33

      Toxic if swallowed.
      Danger of cumulative effects.
      S PhraseS: 37-45

      Wear suitable gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ProteoExtract® Transmembrane Protein Extraction Kit 試験成績書(CoA)

      タイトルロット番号
      71772

      参考資料

      参考資料の概要
      Nakamura, E., et al. 2008. Cancer Sci. 99, 1390.

      カタログ

      タイトル
      Western Blotting Tools
      安定したタンパク質抽出と効率的なサンプル調整に Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品

      引用

      Title
    • Waetzig G.H., et al. 2010. The Journal of Biological Chemistry 285, 1781.
    • Ernst, C.M., et al. 2009. doi: 10.1371/journal.ppat.1000660
    • ユーザープロトコール

      タイトル
      TB477