PvuRts1I (5-hmC Restriction Endonuclease)

35 kDa

PvuRts1I is an enzyme isolated from the gram negative bacterium Proteus vulgaris that can specifically digest DNA containing 5-hmC (Szwagierczak et al., Nucleic Acids Research, 2011) while leaving cytosine and 5-mC intact. This endonuclease can be used to analyze 5-hmC patterns in mammalian genomes by cleave cutting both glucosylated and non-glucosylated 5-hmC containing DNA sequences.

Research studies of DNA methylation and gene expression support the presence of 5-hydroxymethylcytosine (5-hmC) as a sixth base in mammalian genomes. 5-hmC is generated by Ten Eleven Translocation (TET) family of enzymes through oxidation of 5-methylcytosine (5-mC). Chemically, 5-hmC differs from 5-mC by the presence of a hydroxyl on the methyl group at position 5 of cytosine.

Materials Provided:
PvuRts1I 5-hmC Endonuclease: 100U (1U/µL) in a buffer containing 20mM Tris pH 8.0, 150mM NaCl, 1mM DTT and 50% Glycerol.

PvuRts1I 5-hmC 10X Reaction Buffer: 500µL of buffer containing 200mM Tris pH 8.0, 1.5M NaCl, 50mM MgCl2 and 10mM DTT.

Stable at -20°C for 1 year from date of receipt.

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